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The stolbur phytoplasma antigenic membrane protein gene stamp is submitted to diversifying positive selection

hal.structure.identifierGénomique, développement et pouvoir pathogène [GD2P]
dc.contributor.authorFABRE, Anne-Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDANET, Jean-Luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
dc.date.issued2011
dc.identifier.issn0378-1119
dc.description.abstractEnSurface proteins play an important role in phytoplasma life cycle. The antigenic membrane protein (AMP) of ‘Candidatus Phytoplasma asteris’ has been shown to interact with the insect microfilaments. Due to the synteny of the groL-amp-nadE genetic locus between phytoplasma genomes, the gene stamp that encodes the antigenic membrane protein of stolbur phytoplasma has been cloned and characterized. It encodes a 157 amino acid-long protein with a predicted signal peptide and a C-terminal hydrophobic alpha-helix. STAMP was 26–40% identical to AMP of ‘Candidatus Phytoplasma asteris’ strains and 40% identical to AMP of ‘Ca. P. japonicum'. The expression of STAMP in Escherichia. coli produced a 16 kDa peptide recognized by an anti-stolbur monoclonal antibody. Stamp was more variable than the house-keeping gene secY and the ratio between non-synonymous over synonymous mutations (dN/dS ) was 2.78 for stamp as compared to 0.64 for secY. This indicates that stamp is submitted to a positive diversifying selection pressure.
dc.language.isoen
dc.publisherElsevier
dc.subjectMOLLICUTE
dc.subjectSURFACE PROTEIN
dc.subjectBACTERIOLOGIE
dc.subjectGENETIQUE
dc.subjectPROTEINE DE SURFACE
dc.subjectBACTERIE DU PHLOEME
dc.subject.enGENETIC DIVERSITY
dc.subject.enPOSITIVE SELECTION
dc.subject.enPHLOEM-RESTRICTED-BACTERIA
dc.title.enThe stolbur phytoplasma antigenic membrane protein gene stamp is submitted to diversifying positive selection
dc.typeArticle de revue
dc.identifier.doi10.1016/j.gene.2010.10.012
dc.subject.halSciences du Vivant [q-bio]/Génétique
bordeaux.journalGene
bordeaux.page37-41
bordeaux.volume472
bordeaux.issue1-2
bordeaux.peerReviewedoui
hal.identifierhal-02647625
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647625v1
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