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Transgenic plums expressing plum pox virus coat protein gene do not assist the development of virus recombinants under field conditions

hal.structure.identifierFruit Research and Development Station Bistrita
dc.contributor.authorZAGRAI, I.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRAVELONANDRO, Michel
hal.structure.identifierUniversity of Agricultural Sciences and Veterinary Medicine
dc.contributor.authorGABOREANU, I.
hal.structure.identifierMolecular Biology Center Cluj-Napoca
dc.contributor.authorFERENCZ, B.
hal.structure.identifierARS
dc.contributor.authorSCORZA, R.
hal.structure.identifierFruit Research and Development Station Bistrita
dc.contributor.authorZAGRAI, L.
hal.structure.identifierMolecular Biology
dc.contributor.authorKELEMEN, B.
hal.structure.identifierUniversity of Agricultural Sciences and Veterinary Medicine
dc.contributor.authorPAMFIL, D.
hal.structure.identifierMolecular Biology
dc.contributor.authorPOPESCU, O.
dc.date.issued2011
dc.identifier.issn1125-4653
dc.description.abstractEnThe serological and genetic variability of Plum pox virus (PPV) isolates from transgenic plum trees expressing the PPV coat protein gene and conventional plums was analyzed. PPV isolates were characterized serologically by TAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies and by molecular typing across three genomic regions as well as RFLP analysis of the 3' terminus of the CP gene. PCR products spanning the (Cter)CP and (Cter)NIb-(Nter)CP regions were sequenced. Sequence information revealed no significant difference between serological and molecular features of PPV isolates from transgenic and conventional plums that were growing together in experimental orchards for 6-8 years. These results show that transgenic plums do not promote the emergence of new PPV variants under field conditions.
dc.language.isoen
dc.publisherSpringer
dc.subjectGENETIQUE
dc.subject.enPATHOGEN DERIVED RESISTANCE
dc.subject.enGMO
dc.subject.enCP GENE
dc.subject.enSAFETY ISSUE
dc.subject.enRECOMBINANT VIRUSES
dc.title.enTransgenic plums expressing plum pox virus coat protein gene do not assist the development of virus recombinants under field conditions
dc.typeArticle de revue
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalJournal of Plant Pathology
bordeaux.page159-165
bordeaux.volume93
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02648545
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02648545v1
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