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Identification and characterization of tomato mutants affected in the Rx-mediated resistance to PVX isolates

hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorSTURBOIS, Bénédicte
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA-OURABAH, Marie-Pierre
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorGOMBERT, Julie
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorLASSEUR, Bertrand
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorMACQUET, Audrey
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorBENDAHMANE, Abdelhafid
hal.structure.identifierUnité de recherche en génomique végétale [URGV]
dc.contributor.authorBAURÈS, Isabelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2012
dc.identifier.issn0894-0282
dc.description.abstractEnFive tomato mutants affected in the Rx-mediated resistance against Potato virus X (PVX) were identified by screening a mutagenized population derived from a transgenic, Rx1-expressing 'Micro-Tom' line. Contrary to their parental line, they failed to develop lethal systemic necrosis upon infection with the virulent PVX-KH2 isolate. Sequence analysis and quantitative reverse-transcription polymerase chain reaction experiments indicated that the mutants are not affected in the Rx1 transgene or in the Hsp90, RanGap1 and RanGap2, Rar1 and Sgt1 genes. Inoculation with the PVX-CP4 avirulent isolate demonstrated that the Rx1 resistance was still effective in the mutants. In contrast, the virulent PVX-KH2 isolate accumulation was readily detectable in all mutants, which could further be separated in two groups depending on their ability to restrict the accumulation of PVX-RR, a mutant affected at two key positions for Rx1 elicitor activity. Finally, transient expression of the viral capsid protein elicitor indicated that the various mutants have retained the ability to mount an Rx1-mediated hypersensitive response. Taken together, the results obtained are consistent with a modification of the specificity or intensity of the Rx1-mediated response. The five Micro-Tom mutants should provide very valuable resources for the identification of novel tomato genes affecting the functioning of the Rx gene.
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.subjectBIOLOGIE MOLECULAIRE
dc.subjectGENETIQUE
dc.subjectPVX
dc.subject.enMICRO-TOM
dc.subject.enRESISTANCE MEDIEE
dc.title.enIdentification and characterization of tomato mutants affected in the Rx-mediated resistance to PVX isolates
dc.typeArticle de revue
dc.identifier.doi10.1094/MPMI-07-11-0181
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalMolecular Plant-Microbe Interactions
bordeaux.page341-354
bordeaux.volume25
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02649935
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02649935v1
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