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Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries

hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOKABE, Yoshihiro
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorASAMIZU, Erika
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorSAITO, Takeshi
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorMATSUKURA, Chiaki
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorARIIZUMI, Tohru
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorMIZOGUCHI, Tsuyoshi
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
dc.date.issued2011
dc.identifier.issn0032-0781
dc.description.abstractEnTo accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. The 0.5% EMS population had a mild mutation frequency of one mutation per 1,710 kb, whereas the 1.0% EMS population had a frequency of one mutation per 737 kb, a frequency suitable for producing an allelic series of mutations in the target genes. The overall mutation frequency was one mutation per 1,237 kb, which affected an average of three alleles per kilobase screened. To assess whether a Micro-Tom TILLING platform could be used for efficient mutant isolation, six ethylene receptor genes in tomato (SlETR1-SlETR6) were screened. Two allelic mutants of SlETR1 (Sletr1-1 and Sletr1-2) that resulted in reduced ethylene responses were identified, indicating that our Micro-Tom TILLING platform provides a powerful tool for the rapid detection of mutations in an EMS mutant library. This work provides a practical and publicly accessible tool for the study of fruit biology and for obtaining novel genetic material that can be used to improve important agronomic traits in tomato.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectREVERSE GENETICS
dc.subjectBIOLOGIE DU DEVELOPPEMENT
dc.subjectBIOLOGIE VEGETALE
dc.subject.enETHYLENE RECEPTOR
dc.subject.enFRUIT RIPENING
dc.subject.enMICRO-TOM
dc.subject.enTILLING
dc.subject.enTOMATO
dc.title.enTomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries
dc.typeArticle de revue
dc.identifier.doi10.1093/pcp/pcr134
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPlant and Cell Physiology
bordeaux.page1994-2005
bordeaux.volume52
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-02650534
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02650534v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20and%20Cell%20Physiology&rft.date=2011&rft.volume=52&rft.issue=11&rft.spage=1994-2005&rft.epage=1994-2005&rft.eissn=0032-0781&rft.issn=0032-0781&rft.au=OKABE,%20Yoshihiro&ASAMIZU,%20Erika&SAITO,%20Takeshi&MATSUKURA,%20Chiaki&ARIIZUMI,%20Tohru&rft.genre=article


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