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hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorVILAINE, Francoise
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorKERCHEV, Pavel Ivanov
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorCLÉMENT, Gilles
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATAILLER, Brigitte
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorCAYLA, Thibaud
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorBILL, Laurence
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorGISSOT, Lionel
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorDINANT, Sylvie
dc.date.issued2013
dc.identifier.issn1040-4651
dc.description.abstractEnThe complex process of phloem sugar transport involves symplasmic and apoplasmic events. We characterized Arabidopsis thaliana lines ectopically expressing a phloem-specific gene encoding NDR1/HIN1-like26 (NHL26), a putative membrane protein. NHL26 overexpressor plants grew more slowly than wild-type plants, accumulated high levels of carbohydrates in mature leaves, and had a higher shoot biomass, contrasting with slower root growth and a lower seed yield. Similar effects were observed when NHL26 was overexpressed in companion cells, under the control of a companion cell-specific promoter. The soluble sugar content of the phloem sap and sink organs was lower than that in the wild type, providing evidence of a sugar export defect. This was confirmed in a phloem-export assay with the symplastic tracer carboxyfluorescein diacetate. Leaf sugar accumulation was accompanied by higher organic acid, amino acid, and protein contents, whereas analysis of the metabolite profile of phloem sap exudate revealed no change in amino acid or organic acid content, indicating a specific effect on sugar export. NHL26 was found to be located in the phloem plasmodesmata and the endoplasmic reticulum. These findings reveal that NHL26 accumulation affects either the permeability of plasmodesmata or sugar signaling in companion cells, with a specific effect on sugar export.
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.subject.envirus movement protein
dc.subject.enembryogenesis abundant proteins
dc.subject.enpromote carbohydrate export
dc.subject.entissue specific expression
dc.subject.entransgenic potato plants
dc.subject.enmaize leaves
dc.subject.engene expression
dc.subject.enamino acids
dc.subject.enseed yield
dc.subject.ensucrose transporters
dc.title.enIncreased expression of a phloem membrane protein encoded by NHL26 alters phloem export and sugar partitioning in [i]Arabidopsis[/i]
dc.typeArticle de revue
dc.identifier.doi10.1105/tpc.113.111849
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalThe Plant cell
bordeaux.page1689 -1708
bordeaux.volume25
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-01001524
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01001524v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=The%20Plant%20cell&rft.date=2013&rft.volume=25&rft.issue=5&rft.spage=1689%20-1708&rft.epage=1689%20-1708&rft.eissn=1040-4651&rft.issn=1040-4651&rft.au=VILAINE,%20Francoise&KERCHEV,%20Pavel%20Ivanov&CL%C3%89MENT,%20Gilles&BATAILLER,%20Brigitte&CAYLA,%20Thibaud&rft.genre=article


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