Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots
| hal.structure.identifier | Université de Tunis | |
| dc.contributor.author | BOULILA-ZOGHLAMI, Latifa | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | GALLUSCI, Philippe | |
| hal.structure.identifier | University of California [Riverside] [UC Riverside] | |
| dc.contributor.author | HOLZER, Frances M. | |
| hal.structure.identifier | University of Nebraska System | |
| dc.contributor.author | BASSET, Gilles J. | |
| hal.structure.identifier | Université de Tunis | |
| dc.contributor.author | DJEBALI, Whabi | |
| hal.structure.identifier | Université de Tunis | |
| dc.contributor.author | CHAIBI, Wided | |
| hal.structure.identifier | University of Nebraska System | |
| dc.contributor.author | WALLING, Linda L. | |
| hal.structure.identifier | Interactions Biotiques et Santé Végétale [IBSV] | |
| dc.contributor.author | BROUQUISSE, Renaud | |
| dc.date.issued | 2011 | |
| dc.identifier.issn | 0032-0935 | |
| dc.description.abstractEn | The effects of cadmium (Cd) on aminopeptidase (AP) activities and Leucine-AP (LAP) expression were investigated in the roots of tomato (Solanum lycopersicum L., var Ibiza) plants. Three-week-old plants were grown for 10 days in the presence of 0.3-300 μM Cd and compared to control plants grown in the absence of Cd. AP activities were measured using six different p-nitroanilide (p-NA) substrates. Leu, Met, Arg, Pro and Lys hydrolyzing activities increased in roots of Cd-treated plants, while Phe-pNA cleavage was not enhanced after Cd treatments. The use of peptidase inhibitors showed that most of the Leu-pNA hydrolyzing activity was related to one or several metallo-APs. Changes in Lap transcripts, protein and activities were measured in the roots of 0 and 30-μM Cd-treated plants. LapA transcript levels increased in Cd-treated roots, whereas LapN RNAs levels were not modified. To assess amount of Leu-pNA hydrolyzing activity associated with the hexameric LAPs, LAP activity was measured following immunoprecipitation with a LAP polyclonal antiserum. LAP activity increased in Cd-treated roots. There was a corresponding increase in LAP-A protein levels detected in 2D-immunoblots. The role of LAP-A in the proteolytic response to Cd stress is discussed. | |
| dc.language.iso | en | |
| dc.publisher | Springer Verlag | |
| dc.subject | BIOLOGIE VEGETALE | |
| dc.subject.en | LAP-A | |
| dc.title.en | Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1007/s00425-011-1468-y | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Génétique/Génétique des plantes | |
| bordeaux.journal | Planta | |
| bordeaux.page | 857-863 | |
| bordeaux.volume | 234 | |
| bordeaux.issue | 4 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02652540 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02652540v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Planta&rft.date=2011&rft.volume=234&rft.issue=4&rft.spage=857-863&rft.epage=857-863&rft.eissn=0032-0935&rft.issn=0032-0935&rft.au=BOULILA-ZOGHLAMI,%20Latifa&GALLUSCI,%20Philippe&HOLZER,%20Frances%20M.&BASSET,%20Gilles%20J.&DJEBALI,%20Whabi&rft.genre=article |
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