AOUINI, Asma; HERNOULD, Michel; ARIIZUMI, Tohru; MATSUKURA, Chiaki; EZURA, Hiroshi; ASAMIZU, Erika

doi:10.5511/plantbiotechnology.12.0213a

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AOUINI, Asma
Université de Tsukuba = University of Tsukuba

HERNOULD, Michel
Biologie du fruit et pathologie [BFP]

ARIIZUMI, Tohru
Université de Tsukuba = University of Tsukuba

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Article de revue

Ce document a été publié dans

Plant Biotechnology. 2012, vol. 29, n° 3, p. 229 - 235

Japanese Society for Plant Cell and Molecular Biology

Résumé en anglais

Thirteen glutamate receptor-like genes have recently been identified in tomato plants; however, their functions have not been fully elucidated. We overexpressed SlGLR1.1 and SlGLR3.5 in Arabidopsis and found that transgenic plants showed symptoms such as curled and deformed leaves, dwarf stature and retarded growth resembling those of Ca2+ deficiency. The results revealed that the levels of Ca2+ in aerial tissues did not differ between wild-type and transgenic plants, suggesting that overexpression of SlGLR1.1 and SlGLR3.5 did not affect Ca2+ uptake. Transgenic lines were hypersensitive to K+ and Na+ ionic stresses, which was rescued by addition of Ca2+ to the growth medium. Ectopic expression of SlGLR1.1 and SlGLR3.5 resulted in reduced efficiency of Ca2+ utilization, suggesting that these genes may play a role in calcium assimilation in tomato plants by controlling ionic transport across the membrane.< Réduire

Mots clés

PLANTS

EXPRESSION

CULTURES

CELLS

Mots clés en anglais

Glutamate receptor-like

tomato

overexpression

calcium

ion hypersensitivity

ARABIDOPSIS-THALIANA

HOMOLOG