Redox-Immunofunctionalized Potyvirus Nanoparticles for High-Resolution Imaging by AFM-SECM Correlative Microscopy.
| hal.structure.identifier | Université Paris Diderot - Paris 7 [UPD7] | |
| dc.contributor.author | ANNE, Agnès | |
| hal.structure.identifier | Université Paris Diderot - Paris 7 [UPD7] | |
| dc.contributor.author | CHOVIN, Arnaud | |
| hal.structure.identifier | Université Paris Diderot - Paris 7 [UPD7] | |
| dc.contributor.author | DEMAILLE, Christophe | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | MICHON, Thierry | |
| dc.date.issued | 2018 | |
| dc.identifier.isbn | 978-1-4939-7806-9 | |
| dc.description.abstractEn | We present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence of ferrocene functionalized specific antibodies on the surface of potyvirus particles. For this purpose, potyviruses, flexuous filamentous phytoviruses with a high aspect ratio, have been chosen. This technique allows analysis of the distribution of antibody labeling over the virus population. But, more importantly, it opens up the imaging of immune complexes decorating a single viral particle. Finally, its high resolution allows the characterization in situ of the ultrastructure of a single immune complex on the particle. | |
| dc.language.iso | en | |
| dc.publisher | Humana Press | |
| dc.publisher.location | New York (united states) | |
| dc.source.title | Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols | |
| dc.subject | Electrochemical atomic force microscopy | |
| dc.subject | Lettuce mosaic virus (LMV) | |
| dc.subject.en | Potyvirus | |
| dc.subject.en | Redox antibodies | |
| dc.subject.en | Single viral protein imaging | |
| dc.subject.en | Viral nanoparticles | |
| dc.title.en | Redox-Immunofunctionalized Potyvirus Nanoparticles for High-Resolution Imaging by AFM-SECM Correlative Microscopy. | |
| dc.type | Chapitre d'ouvrage | |
| dc.identifier.doi | 10.1007/978-1-4939-7808-3_30 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
| dc.subject.hal | Sciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique | |
| bordeaux.volume | 1776 | |
| bordeaux.title.proceeding | Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols | |
| hal.identifier | hal-02791110 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02791110v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Virus-Derived%20Nanoparticles%20for%20Advanced%20Technologies%20:%20Methods%20and%20Protocols&rft.date=2018&rft.volume=1776&rft.au=ANNE,%20Agn%C3%A8s&CHOVIN,%20Arnaud&DEMAILLE,%20Christophe&MICHON,%20Thierry&rft.isbn=978-1-4939-7806-9&rft.genre=unknown |
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