Analysis of enzyme activities
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MENARD, Guillaume | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BIAIS, Benoit | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | PRODHOMME, Duyên | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BALLIAS, Patricia | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GIBON, Yves | |
dc.date.issued | 2014 | |
dc.identifier.isbn | 978-1-62703-687-0 | |
dc.description.abstractEn | The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented. | |
dc.language.iso | en | |
dc.publisher | Springer Science+Business Media | |
dc.publisher.location | New York (united states) | |
dc.source.title | Plant Metabolic Flux Analysis : Methods and Protocols | |
dc.subject | microplate | |
dc.subject.en | enzyme activity | |
dc.subject.en | direct assay | |
dc.subject.en | stopped assay | |
dc.subject.en | cycling assay | |
dc.title.en | Analysis of enzyme activities | |
dc.type | Chapitre d'ouvrage | |
dc.identifier.doi | 10.1007/978-1-62703-688-7_15 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
bordeaux.volume | 1090 | |
bordeaux.title.proceeding | Plant Metabolic Flux Analysis : Methods and Protocols | |
hal.identifier | hal-02799530 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02799530v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Plant%20Metabolic%20Flux%20Analysis%20:%20Methods%20and%20Protocols&rft.date=2014&rft.volume=1090&rft.au=MENARD,%20Guillaume&BIAIS,%20Benoit&PRODHOMME,%20Duy%C3%AAn&BALLIAS,%20Patricia&GIBON,%20Yves&rft.isbn=978-1-62703-687-0&rft.genre=unknown |
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