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hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMENARD, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoit
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRODHOMME, Duyên
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALLIAS, Patricia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
dc.date.issued2014
dc.identifier.isbn978-1-62703-687-0
dc.description.abstractEnThe evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented.
dc.language.isoen
dc.publisherSpringer Science+Business Media
dc.publisher.locationNew York (united states)
dc.source.titlePlant Metabolic Flux Analysis : Methods and Protocols
dc.subjectmicroplate
dc.subject.enenzyme activity
dc.subject.endirect assay
dc.subject.enstopped assay
dc.subject.encycling assay
dc.title.enAnalysis of enzyme activities
dc.typeChapitre d'ouvrage
dc.identifier.doi10.1007/978-1-62703-688-7_15
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.volume1090
bordeaux.title.proceedingPlant Metabolic Flux Analysis : Methods and Protocols
hal.identifierhal-02799530
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02799530v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Plant%20Metabolic%20Flux%20Analysis%20:%20Methods%20and%20Protocols&rft.date=2014&rft.volume=1090&rft.au=MENARD,%20Guillaume&BIAIS,%20Benoit&PRODHOMME,%20Duy%C3%AAn&BALLIAS,%20Patricia&GIBON,%20Yves&rft.isbn=978-1-62703-687-0&rft.genre=unknown


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