Tuf and secY PCR amplification and genotyping of phytoplasmas
Langue
en
Chapitre d'ouvrage
Ce document a été publié dans
Phytoplasma: Methods and Protocols, Phytoplasma: Methods and Protocols. 2013, vol. 938
Springer Science+Business Media
Résumé en anglais
Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. ...Lire la suite >
Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence.< Réduire
Mots clés
Bacterial Proteins/ genetics
Epidemiology
tuf
Mots clés en anglais
Genetic diversity
Polymerase chain reaction
Phylogenetic analysis
secY
Origine
Importé de halUnités de recherche