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Molecular characterization of a sadwavirus and a potexvirus infecting yams (Dioscorea spp) in Guadeloupe

hal.structure.identifierAgrosystèmes tropicaux [ASTRO]
dc.contributor.authorANZALA, Fabiola
hal.structure.identifierBiologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorFILLOUX, Denis
hal.structure.identifierAgrosystèmes tropicaux [ASTRO]
dc.contributor.authorGOMEZ, Rose-Marie
hal.structure.identifierBiologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorROUMAGNAC, Philippe
hal.structure.identifierAgrosystèmes tropicaux [ASTRO]
dc.contributor.authorPAVIS, Claude
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorTEYCHENEY, Pierre-Yves
dc.date.issued2011
dc.date.conference2011-09-11
dc.description.abstractEnYams (Dioscorea sp.) are important food commodities in tropical and subtropical areas of Africa, the Caribbean, South America and the Pacific, where they play a key role in food security. Being vegetatively propagated crops, yams cannot be sanitized via seed production and therefore accumulate viruses over long periods of time. Potexvirus, Carlavirus, Badnavirus, Cucumovirus, Macluravirus and Potyvirus species have been characterized in yams. In addition ESTs (Expressed Sequence Tags) of Dioscorea alata include sequences from families of viruses yet unknown to infect yams, such as Geminiviridae and Secoviridae, showing that the diversity of viral species infecting this crop remains underestimated. This situation has prompted a search for yet uncharacterized viruses infecting yams in Guadeloupe (French West Indies). RT-PCR was performed on crude extracts of symptomatic yams (D. alata, D. trifida), following direct binding of viral particles and using Potexvirus degenerate primers [1]. Several samples gave amplification products, which were cloned and sequenced. Some of them displayed significant levels of homology with Potexviruses and Sadwaviruses. The 3’ ends of the corresponding viral genomes have been amplified by 3’ RACE, cloned and sequenced. Phylogenetic analyses confirm the existence in yams of a new viral species within the genus Potexvirus, tentatively named Yam virus X (YVX), and of a new viral species within the genus Sadwavirus, tentatively named Yam necrosis virus (YNV).
dc.language.isoen
dc.title.enMolecular characterization of a sadwavirus and a potexvirus infecting yams (Dioscorea spp) in Guadeloupe
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
dc.subject.halSciences de l'Homme et Société
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Ingénierie des aliments
dc.subject.halSciences de l'ingénieur [physics]/Génie des procédés
bordeaux.conference.title15. International Congress of Virology (IUMS 2011 Sapporo)
bordeaux.countryJP
bordeaux.conference.citySapporo
bordeaux.peerReviewedoui
hal.identifierhal-02808097
hal.version1
hal.invitednon
hal.conference.end2011-09-16
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02808097v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2011&rft.au=ANZALA,%20Fabiola&FILLOUX,%20Denis&GOMEZ,%20Rose-Marie&ROUMAGNAC,%20Philippe&PAVIS,%20Claude&rft.genre=conference


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