MUSSEAU, Constance; JORLY, Joana; GADIN, Stéphanie; SØRENSEN, Iben; DEBORDE, Catherine; BERNILLON, Stéphane; MAUXION, Jean-Philippe; ATIENZA, Isabelle; MOING, Annick; LEMAIRE-CHAMLEY, Martine; ROSE, Jocelyn K.C.; CHEVALIER, Christian; ROTHAN, Christophe; FERNANDEZ-LOCHU, Lucie; GÉVAUDANT, Frederic

doi:10.1105/tpc.20.00245

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MUSSEAU, Constance
Biologie du fruit et pathologie [BFP]

JORLY, Joana
Biologie du fruit et pathologie [BFP]

GADIN, Stéphanie
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

The Plant cell. 2020, vol. 32, n° 10, p. 3188-3205

American Society of Plant Biologists (ASPB)

Résumé en anglais

Cell fate maintenance is an integral part of plant cell differentiation and the production of functional cells, tissues, and organs. Fleshy fruit development is characterized by the accumulation of water and solutes in the enlarging cells of parenchymatous tissues. In tomato (Solanum lycopersicum), this process is associated with endoreduplication in mesocarp cells. The mechanisms that preserve this developmental program, once initiated, remain unknown. We show here that analysis of a previously identified tomato ethyl methanesulfonate-induced mutant that exhibits abnormal mesocarp cell differentiation could help elucidate determinants of fruit cell fate maintenance. We identified and validated the causal locus through mapping-by-sequencing and gene editing, respectively, and performed metabolic, cellular, and transcriptomic analyses of the mutant phenotype. The data indicate that disruption of the SlGBP1 gene, encoding GUANYLATE BINDING PROTEIN1, induces early termination of endoreduplication followed by late divisions of polyploid mesocarp cells, which consequently acquire the characteristics of young proliferative cells. This study reveals a crucial role of plant GBPs in the control of cell cycle genes, and thus, in cell fate maintenance. We propose that SlGBP1 acts as an inhibitor of cell division, a function conserved with the human hGBP-1 protein.< Réduire

Mots clés

Tomate

Solanum lycopersicum

Division cellulaire

Endoreduplication

Développement du fruit

Fruit charnu

DOI

http://dx.doi.org/10.1105/tpc.20.00245

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332