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Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity

hal.structure.identifierHatay Mustafa Kemal University
dc.contributor.authorCAGLAYAN, Kadriye
hal.structure.identifierHatay Mustafa Kemal University
dc.contributor.authorGAZEL, Mona
hal.structure.identifierUniversity of Maragheh
dc.contributor.authorROUMI, Vahid
hal.structure.identifierHatay Mustafa Kemal University
dc.contributor.authorKOCABAG, Hamide Deniz
hal.structure.identifierHatay Mustafa Kemal University
dc.contributor.authorTUNÇ, Bahar
hal.structure.identifierAgroscope
dc.contributor.authorREYNARD, Jean Sebastien
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorRUIZ-GARCÍA, Ana Belén
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorOLMOS, Antonio
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2020
dc.identifier.issn2073-4395
dc.description.abstractEnPomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0-86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectVirus phytopathogène
dc.subjectVirologie végétale
dc.subject.enPunica granatum
dc.subject.enpotyviridae
dc.subject.enHTS
dc.subject.enelectron microscopy
dc.subject.enRT-PCR
dc.title.enIdentification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity
dc.typeArticle de revue
dc.identifier.doi10.3390/agronomy10111821
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalAgronomy
bordeaux.page1821
bordeaux.volume10
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-03033480
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03033480v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Agronomy&rft.date=2020&rft.volume=10&rft.issue=11&rft.spage=1821&rft.epage=1821&rft.eissn=2073-4395&rft.issn=2073-4395&rft.au=CAGLAYAN,%20Kadriye&GAZEL,%20Mona&ROUMI,%20Vahid&KOCABAG,%20Hamide%20Deniz&TUN%C3%87,%20Bahar&rft.genre=article


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