Characterization of the Mycovirome of the Phytopathogenic Fungus, Neofusicoccum parvum
Language
en
Article de revue
This item was published in
Viruses. 2021, vol. 13, n° 3, p. 375
MDPI
English Abstract
Neofusicoccum parvum is a fungal plant-pathogen belonging to the family Botryosphaeriaceae, and is considered one of the most aggressive causal agents of the grapevine trunk disease (GTD) Botryosphaeria dieback. In this ...Read more >
Neofusicoccum parvum is a fungal plant-pathogen belonging to the family Botryosphaeriaceae, and is considered one of the most aggressive causal agents of the grapevine trunk disease (GTD) Botryosphaeria dieback. In this study, the mycovirome of a single strain of N. parvum (COLB) was characterized by high throughput sequencing analysis of total RNA and subsequent bioinformatic analyses. Contig annotations, genome completions, and phylogenetic analyses allowed us to describe six novel mycoviruses belonging to four different viral families. The virome is composed of two victoriviruses in the family Totiviridae, one alphaendornavirus in the family Endornaviridae, two mitoviruses in the family Mitoviridae, and one narnavirus belonging to the family Narnaviridae. The presence of the co-infecting viruses was confirmed by sequencing the RT-PCR products generated from total nucleic acids extracted from COLB. This study shows that the mycovirome of a single N. parvum strain is highly diverse and distinct from that previously described in N. parvum strains isolated from grapevines.Read less <
Keywords
Pathologie végétale
virus phytopathogène
virologie végétale
English Keywords
Botryosphaeriaceae
Neofusicoccum parvum
fungi
grapevine trunk disease
high throughput sequencing
mycovirus
ANR Project
COntinental To coastal Ecosystems: evolution, adaptability and governance - ANR-10-LABX-0045
Origin
Hal importedCollections