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Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARRICAU-BOUVERY, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA, Marie-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDESQUÉ, Delphine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorEVEILLARD, Sandrine
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorBROCARD, Lysiane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
dc.date.issued2021-05-27
dc.identifier.issn2045-2322
dc.description.abstractEnThe flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectflavescence dorée
dc.subjectMollicute phytopathogène
dc.subjectphytoplasme
dc.subjectbactérie phytopathogène
dc.subjectinsecte vecteur
dc.subjectinteraction
dc.subjectpathologie végétale
dc.subjectsanté des plantes
dc.subjectScaphoideus titanus
dc.subjectEuscelidius variegatus
dc.subject.enadhesin Vmpa
dc.title.enInteractions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-021-90809-z
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalScientific Reports
bordeaux.page11222
bordeaux.volume11
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03244281
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03244281v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2021-05-27&rft.volume=11&rft.issue=1&rft.spage=11222&rft.epage=11222&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=ARRICAU-BOUVERY,%20Nathalie&DURET,%20Sybille&DUBRANA,%20Marie-Pierre&DESQU%C3%89,%20Delphine&EVEILLARD,%20Sandrine&rft.genre=article


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