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Thioredoxin m overexpression in chloroplasts alters carbon and nitrogen partitioning in tobacco plants

hal.structure.identifierUniversidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]
dc.contributor.authorANCÍN, María
hal.structure.identifierUniversidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]
dc.contributor.authorLARRAYA, Luis
hal.structure.identifierMax-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
dc.contributor.authorFLOREZ-SARASA, Igor
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierUniversidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]
dc.contributor.authorFERNÁNDEZ-SAN MILLÁN, Alicia
hal.structure.identifierUniversidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]
dc.contributor.authorVERAMENDI, Jon
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierMax-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
dc.contributor.authorFERNIE, Alisdair
hal.structure.identifierConsejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] [CSIC]
dc.contributor.authorARANJUELO, Iker
hal.structure.identifierUniversidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]
dc.contributor.authorFARRAN, Inmaculada
dc.date.issued2021
dc.identifier.issn0022-0957
dc.description.abstractEnIn plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism and its coordination is fundamental for plant growth and development. In the present work, the influence of thioredoxin (Trx) m on C and N partitioning was studied using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants, indicating an up-regulation of the GS-GOGAT pathway. Trx m-overexpressing plants specifically displayed increased activity and stability of glutamine synthetase in tobacco plants. Moreover, higher photorespiration and nitrate accumulation were determined in these plants relative to the untransformed control, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, the combined results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectMetabolisme
dc.subjectPlante
dc.subject.enGS-GOGAT pathway
dc.subject.enThioredoxin
dc.subject.encarbon metabolism
dc.subject.enchloroplast
dc.subject.englutamine synthetase
dc.subject.ennitrogen metabolism
dc.subject.enphotorespiration
dc.title.enThioredoxin m overexpression in chloroplasts alters carbon and nitrogen partitioning in tobacco plants
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erab193
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Experimental Botany
bordeaux.page4949-4964
bordeaux.volume72
bordeaux.issue13
bordeaux.peerReviewedoui
hal.identifierhal-03224310
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03224310v1
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