Sweet cherry (Prunus avium L.) is a diploid species with an estimated genome size of 338 MB (Arumuganathan et al. 1991). It is mostly selfincompatible, and therefore has a heterozygous genetic background. We have sequenced the genome of the 'Regina' sweet cherry variety, which is a late blooming cultivar. Here we present whole-chromosomes assembly and phasing using a combination of long read sequencing, proximity ligation (HiC) data and GBS markers.Regina PacBio long reads were de novo assembled using FALCON UNZIP. A first assembly using optical mapping and high density genetic linkage maps with GBS data resulted in a genome of 264,8 Mb (78,4 % of estimated genome size) distributed on 8 linkage groups with a scaffold N50~6Mb (Regina V1). A new assembly (ReginaV2) combining Falcon Unzip assembly of PacBio long reads, proximity ligation (HiC) data and GBS markers has been done with Falcon Phased and the Proximo pipeline of Phase Genomics. It produced complete, fully phased, diploid chromosome scale scaffolds with a genome coverage of~90%, a N50 of~37 Mb and a complete BUSCO score of 95,6%. Structural and functional annotations of Regina V2 are in progress. Obtaining these phased chromosomes will allow very detailed studies of the allelic effects on the traits studied, in particular by making it possible to study the allelic expressions of candidate genes.< Leer menos