A Chimeric TGA Repressor Slows Down Fruit Maturation and Ripening in Tomato
YOSHIDA, Takuya
The University of Tokyo [UTokyo]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
The University of Tokyo [UTokyo]
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
HAJIREZAEI, Mohammad-Reza
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
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Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Langue
en
Article de revue
Ce document a été publié dans
Plant and Cell Physiology. 2022, vol. 63, n° 1, p. 120–134
Oxford University Press (OUP)
Résumé en anglais
Abstract The bZIP transcription factor (TF) SlTGA2.2 was previously highlighted as a possible hub in a network regulating fruit growth and transition to ripening (maturation phase). It belongs to a clade of transcription ...Lire la suite >
Abstract The bZIP transcription factor (TF) SlTGA2.2 was previously highlighted as a possible hub in a network regulating fruit growth and transition to ripening (maturation phase). It belongs to a clade of transcription factors well known for their involvement in the regulation of the salicylic acid (SA) – dependent systemic acquired resistance (SAR). To investigate if this TGA TF plays a role in tomato fruit growth and maturation, we took advantage of the fruit-specific SlPPC2 promoter (PPC2pro) to target the expression of a SlTGA2.2-SRDX chimeric repressor in a developmental window restricted to early fruit growth and maturation. Here, we show that this SlTGA2.2-SRDX repressor alters early fruit development and metabolism, including chloroplast number and structure, considerably extends the time necessary to reach the mature green stage and slows down fruit ripening. RNAseq and plant hormone analyses reveal that PPC2pro:SlTGA2.2-SRDX fruits are maintained in an immature stage as long as PPC2pro is active, through early modifications of plant hormonal signalling and down-regulation of MADS-RIN and NAC-NOR ripening regulators. Once PPC2pro becomes inactive and therefore SlTGA2.2-SRDX expression is reduced, ripening can proceed, albeit at a slower pace than normal. Altogether, this work emphasizes the developmental continuum between fruit growth, maturation and ripening and provides a useful tool to alter and study the molecular bases of tomato fruit transition to ripening.< Réduire
Mots clés
Fruit
développement du fruit
Mots clés en anglais
bZIP
chloroplast
hormone regulation
ripening competency
Solanum lycopersicum
tomato
Origine
Importé de halUnités de recherche