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Model-driven design allows growth of Mycoplasma pneumoniae on serum-free media

hal.structure.identifierLaboratory of Systems and Synthetic Biology
dc.contributor.authorGASPARI, Erika
hal.structure.identifierLaboratory of Systems and Synthetic Biology
dc.contributor.authorMALACHOWSKI, Antoni
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGARCIA-MORALES, Luis
hal.structure.identifierThe Barcelona Institute for Science and Technology [Barcelona, Spain]
dc.contributor.authorBURGOS, Raul
hal.structure.identifierThe Barcelona Institute for Science and Technology [Barcelona, Spain]
dc.contributor.authorSERRANO, Luis
hal.structure.identifierLaboratory of Systems and Synthetic Biology
dc.contributor.authorMARTINS DOS SANTOS, Vitor
hal.structure.identifierLaboratory of Systems and Synthetic Biology
dc.contributor.authorSUAREZ-DIEZ, Maria
dc.date.issued2020-10-23
dc.description.abstractEnMycoplasma pneumoniae is a slow-growing, human pathogen that causes atypical pneumonia. Because it lacks a cell wall, many antibiotics are ineffective. Due to its reduced genome and dearth of many biosynthetic pathways, this fastidious bacterium depends on rich, undefined medium for growth, which makes large-scale cultivation challenging and expensive. To understand factors limiting growth, we developed a genome-scale, constraint-based model of M. pneumoniae called iEG158_mpn to describe the metabolic potential of this bacterium. We have put special emphasis on cell membrane formation to identify key lipid components to maximize bacterial growth. We have used this knowledge to predict essential components validated with in vitro serum-free media able to sustain growth. Our findings also show that glycolysis and lipid metabolism are much less efficient under hypoxia; these findings suggest that factors other than metabolism and membrane formation alone affect the growth of M. pneumoniae. Altogether, our modelling approach allowed us to optimize medium composition, enabled growth in defined media and streamlined operational requirements, thereby providing the basis for stable, reproducible and less expensive production.
dc.language.isoen
dc.publisherNature Research
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enModel-driven design allows growth of Mycoplasma pneumoniae on serum-free media
dc.typeArticle de revue
dc.identifier.doi10.1038/s41540-020-00153-7
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animal
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie
dc.description.sponsorshipEuropeEngineering of Mycoplasma pneumoniae as a broad-spectrum animal vaccine
dc.description.sponsorshipEuropeEngineering of a minimal bacterial therapeutic chassis
bordeaux.journalnpj Systems Biology and Applications
bordeaux.page33
bordeaux.volume6
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03454474
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03454474v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=npj%20Systems%20Biology%20and%20Applications&rft.date=2020-10-23&rft.volume=6&rft.issue=1&rft.spage=33&rft.epage=33&rft.au=GASPARI,%20Erika&MALACHOWSKI,%20Antoni&GARCIA-MORALES,%20Luis&BURGOS,%20Raul&SERRANO,%20Luis&rft.genre=article


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