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hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorIPOUTCHA, Thomas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Géraldine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
dc.date.issued2022
dc.identifier.issn2161-5063
dc.description.abstractEnMycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of Mgal virulence and host specificity are still largely unknown, mostly due to the absence of efficient methods for functional genomics. After evaluating two exogenous recombination systems derived from phages found in the phylogenetically related Spiroplasma phoeniceum and the more distant Bacillus subtilis, the RecET-like system from B. subtilis was successfully used for gene inactivation and targeted replacement in Mgal. In a second step, the Cre-lox recombination system was used for the removal of the antibiotic resistance marker in recombinant mutants. This study therefore describes the first genetic tool for targeted genome engineering of Mgal and demonstrates the efficiency of heterologous recombination systems in minimal bacteria.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subject.enMollicutes
dc.subject.enMycoplasma gallisepticum
dc.subject.enRecET
dc.subject.enmutagenesis
dc.subject.enpoultry
dc.subject.enrecombination
dc.title.enGenome Engineering in Mycoplasma gallisepticum Using Exogenous Recombination Systems
dc.typeArticle de revue
dc.identifier.doi10.1021/acssynbio.1c00541
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalACS Synthetic Biology
bordeaux.page1060-1067
bordeaux.volume11
bordeaux.peerReviewedoui
hal.identifierhal-03583293
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03583293v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Synthetic%20Biology&rft.date=2022&rft.volume=11&rft.spage=1060-1067&rft.epage=1060-1067&rft.eissn=2161-5063&rft.issn=2161-5063&rft.au=IPOUTCHA,%20Thomas&GOURGUES,%20G%C3%A9raldine&LARTIGUE,%20Carole&BLANCHARD,%20Alain&SIRAND-PUGNET,%20Pascal&rft.genre=article


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