BONNOT, Titouan; MARTRE, Pierre; HATTE, Victor; DARDEVET, Mireille; LEROY, Philippe; BENARD, Camille; FALAGAN, Natalia; MARTIN-MAGNIETTE, Marie-Laure; DEBORDE, Catherine; MOING, Annick; GIBON, Yves; PAILLOUX, Marie; BANCEL, Emmanuelle; RAVEL, Catherine

doi:10.1104/pp.19.00842

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Licence d’utilisation du document

BONNOT, Titouan
Génétique Diversité et Ecophysiologie des Céréales [GDEC]
University of California [Riverside] [UC Riverside]

MARTRE, Pierre
Génétique Diversité et Ecophysiologie des Céréales [GDEC]
Écophysiologie des Plantes sous Stress environnementaux [LEPSE]

HATTE, Victor
Génétique Diversité et Ecophysiologie des Céréales [GDEC]

Langue

Article de revue

Ce document a été publié dans

Plant Physiology. 2020-06, vol. 183, n° 2, p. 501-516

Oxford University Press ; American Society of Plant Biologists

Résumé en anglais

Understanding the molecular mechanisms controlling the accumulation of grain storage proteins in response to nitrogen (N) and sulfur (S) nutrition is essential to improve cereal grain nutritional and functional properties. Here, we studied the grain transcriptome and metabolome responses to postanthesis N and S supply for the diploid wheat einkorn (Triticum monococcum). During grain filling, 848 transcripts and 24 metabolites were differentially accumulated in response to N and S availability. The accumulation of total free amino acids per grain and the expression levels of 241 genes showed significant modifications during most of the grain filling period and were upregulated in response to S deficiency. Among them, 24 transcripts strongly responded to S deficiency and were identified in coexpression network analyses as potential coordinators of the grain response to N and S supply. Sulfate transporters and genes involved in sulfate and Met metabolism were upregulated, suggesting regulation of the pool of free amino acids and of the grain N-to-S ratio. Several genes highlighted in this study might limit the impact of S deficiency on the accumulation of grain storage proteins.Sulfur deficiency in einkorn grain leads to upregulation of genes acting in sulfur transport and metabolism that may coordinate the pool of free amino acids necessary for storage protein synthesis.< Réduire

Mots clés

Composition de la protéine du grain

Triticum monococcum

Métabolome

Transcriptome

Réseau de régulation

Mots clés en anglais

Einkorn (Triticum monococcum)

Grain protein composition

Metabolome

Nitrogen 69 and sulphur nutrition

Transcriptome

Regulatory networks

DOI

http://dx.doi.org/10.1104/pp.19.00842

Projet Européen

Wheat and barley Legacy for Breeding Improvement

Project ANR

Développer de nouvelles variétés de blé pour une agriculture durable - ANR-10-BTBR-0003
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Saclay Plant Sciences - ANR-10-LABX-0040

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332