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hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorBEN ABDALLAH, Zeineb
hal.structure.identifierUniversité de Monastir - University of Monastir [UM]
dc.contributor.authorSGHAIER, Halim
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGAMMOUDI, Ibtissem
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMOROTE, F.
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCASSAGNÈRE, Sébastien
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorROMO, Lena
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
dc.date.issued2022-04
dc.identifier.issn2227-9040
dc.description.abstractEnThis work describes the complete elaboration of an immunosensor for the detection of the fungal B1 aflatoxin (AFB1). In a first step, a system made of three screen-printed electrodes (SPEs) was manufactured using gold, silver/silver chloride, and carbon pastes. Raman spectroscopy showed that the thermal treatment applied to the electrodes enabled a strong decrease in the amount of undesirable organic molecules for each paste. Atomic Force Microscopy was also used to reveal the morphology of the electrode surfaces. In a second step, an autonomous and cheap electronic system was designed for the control of the sensor and electrochemical measurements, showing current variations significantly higher than those observed with a commercial system. In a last step, the gold working electrode of this system was functionalized by a simple self-assembly method, optimized in a previous work, with a molecular architecture including an antibody recognizing specifically AFB1. The complete device was finally realized by combining the SPEs and the electronic platform. The resulting setup was able to detect AFB1 toxin in a buffer with an LOD of about 50 fg/mL. It was then applied to the detection of AFB1 in rice milk, a more realistic medium comparable with those met in an agrifood context. The electrochemical detection of AFB1 was possible in a range of concentration between 0.5 pg/mL and 2.5 pg/mL, with the sensor behaving linearly in this range.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enAFB1 toxin
dc.subject.enscreen printing
dc.subject.enelectrochemical immunosensor
dc.subject.enelectronics
dc.subject.enBen Abdallah
dc.subject.enZ.
dc.subject.enSghaier
dc.subject.enH.
dc.subject.enGammoudi
dc.subject.enI.
dc.subject.enMoroté
dc.subject.enF.
dc.subject.enCassagnère
dc.subject.enS.
dc.subject.enRomo
dc.subject.enL.
dc.subject.enBéven
dc.subject.enGrauby-Heywang
dc.subject.enC.
dc.subject.enCohen-Bouhacina
dc.subject.enT. Design
dc.subject.enElaboration
dc.subject.enand Characterization of Chemosensors 2022
dc.subject.en10
dc.subject.en137 AFB1 toxin
dc.title.enDesign, Elaboration, and Characterization of an Immunosensor for the Detection of a Fungal Toxin in Foodstuff Analyses
dc.typeArticle de revue
dc.identifier.doi10.3390/chemosensors10040137
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalChemosensors
bordeaux.page137
bordeaux.volume10
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-03764767
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03764767v1
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