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Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode

hal.structure.identifierLaboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA]
dc.contributor.authorBRAHAM, Yosra
hal.structure.identifierLaboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA]
dc.contributor.authorBARHOUMI, Houcine
hal.structure.identifierLaboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA]
dc.contributor.authorMAAREF, Abderrazak
hal.structure.identifierLaboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir] [LATVPEP]
dc.contributor.authorBAKHROUF, Amina
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
hal.structure.identifierSciences Analytiques [SA]
dc.contributor.authorJAFFREZIC-RENAULT, Nicole
dc.date.created2014-06-28
dc.date.issued2015
dc.identifier.issn2155-6210
dc.description.abstractEnIn this work we describe a new urea biosensor, based on the immobilization of bacteria, Proteus mirabilis on gold electrode. To improve the stability of the bio-system, additional materials were used such as functionalized Fe 3 O 4 nanoparticles (NPs), cationic (PAH), anionic (PSS) polyelectrolytes, Bovine Serum Albumin (BSA) and glutaralde-hyde as a cross-linking agent. The electrochemical performances of the developed bacteria biosensor was evaluated using the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry measurements. The adhesion of the bacteria cell on gold electrode was evaluated using contact angle measurements. The morphology of bacteria and its interaction with Fe 3 O 4 nanoparticles were evaluated with the atomic force microscopy (AFM). As a result, a sensitive, stable and reproducible urea biosensor was developed.
dc.language.isoen
dc.publisherHilaris
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enProteus mirabilis
dc.subject.enFe3O4
dc.subject.enPAH and PSS polyelectrolytes
dc.subject.enImpedance spectroscopy
dc.subject.enGold electrodes
dc.subject.enBacterial adhesion
dc.subject.enAtomic Force Microscopy
dc.title.enCharacterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode
dc.typeArticle de revue
dc.identifier.doi10.4172/2155-6210.1000160
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halChimie/Chimie analytique
bordeaux.journalJournal of Biosensors & Bioelectronics
bordeaux.page1000160
bordeaux.volume6
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-01406863
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01406863v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biosensors%20&%20Bioelectronics&rft.date=2015&rft.volume=6&rft.issue=1&rft.spage=1000160&rft.epage=1000160&rft.eissn=2155-6210&rft.issn=2155-6210&rft.au=BRAHAM,%20Yosra&BARHOUMI,%20Houcine&MAAREF,%20Abderrazak&BAKHROUF,%20Amina&GRAUBY-HEYWANG,%20Christine&rft.genre=article


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