Single metallic nanoparticle imaging for protein detection in cells
Language
en
Article de revue
This item was published in
Proceedings of the National Academy of Sciences of the United States of America. 2003-09-30, vol. 100, n° 20, p. 11350
National Academy of Sciences
English Abstract
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability ...Read more >
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.Read less <
English Keywords
single molecule detection
gold nanoparticle
photothermal
Origin
Hal imported