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Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope

hal.structure.identifierÉcole normale supérieure de Lyon [ENS de Lyon]
dc.contributor.authorSTREPPA, L.
hal.structure.identifierUniversité Claude Bernard Lyon 1 [UCBL]
dc.contributor.authorBERGUIGA, L.
hal.structure.identifierÉcole normale supérieure de Lyon [ENS de Lyon]
dc.contributor.authorBOYER PROVERA, E.
hal.structure.identifierUniversité Claude Bernard Lyon 1 [UCBL]
dc.contributor.authorRATTI, F.
hal.structure.identifierUniversité Claude Bernard Lyon 1 [UCBL]
dc.contributor.authorGOILLOT, E.
hal.structure.identifierÉcole normale supérieure de Lyon [ENS de Lyon]
dc.contributor.authorMARTINEZ TORRES, C.
hal.structure.identifierUniversité Claude Bernard Lyon 1 [UCBL]
dc.contributor.authorSCHAEFFER, L.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorELEZGARAY, J.
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARNEODO, A.
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARGOUL, Françoise
dc.date.issued2016-03-07
dc.date.conference2016-04-22
dc.description.abstractEnWe introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer provides both enhanced sensitivity and long term stability. This microscope takes advantage of the plasmon resonance excitation and the amplification of the electromagnetic field in near-field distance to the gold coated coverslip. This plasmon enhanced evanescent wave microscopy is particularly attractive for the study of cell adhesion and motility since it can be operated without staining of the biological sample. We show that this microscope allows very long-term imaging of living samples, and that it can capture and follow the temporal deformation of C2C12 myoblast cell protusions (lamellipodia), during their migration on a at surface.
dc.language.isoen
dc.publisherSpie-Int Soc Optical Engineering
dc.subject.enDielectric polarisation
dc.subject.enSurface plasmons
dc.subject.enMicroscopy
dc.subject.enPolarisation
dc.subject.enInterferometers
dc.subject.enDielectrics
dc.subject.enHeterodyning
dc.title.enTracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope
dc.typeCommunication dans un congrès
dc.identifier.doi10.1117/12.2211331
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.page97240G (1-10)
bordeaux.volume9724
bordeaux.countryUS
bordeaux.conference.citySan Francisco, CA
bordeaux.peerReviewednon
hal.identifierhal-01556052
hal.version1
hal.invitednon
hal.proceedingsoui
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01556052v1
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