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hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARBONA, Jean-Michel
hal.structure.identifierRégulation transcriptionnelle des génomes [GTR]
hal.structure.identifierLaboratoire de Biologie Intégrative [LBI]
dc.contributor.authorGOLDAR, Arach
hal.structure.identifierInstitut de biologie de l'ENS Paris [IBENS]
dc.contributor.authorHYRIEN, Olivier
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorARNEODO, Alain
hal.structure.identifierLaboratoire de Physique de l'ENS Lyon [Phys-ENS]
dc.contributor.authorAUDIT, Benjamin
dc.date.issued2018-06-01
dc.description.abstractEnThe time-dependent rate I(t) of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firing factors. Here, we show that a normal diffusion of replication fork components towards localized potential replication origins (p-oris) can more simply account for the I(t) universal bell shape, as a consequence of a competition between the origin firing time and the time needed to replicate DNA separating two neighboring p-oris. We predict the I(t) maximal value to be the product of the replication fork speed with the squared p-ori density. We show that this relation is robustly observed in simulations and in experimental data for several eukaryotes. Our work underlines that fork-component recycling and potential origins localization are sufficient spatial ingredients to explain the universality of DNA replication kinetics.
dc.language.isoen
dc.publishereLife Sciences Publication
dc.title.enThe eukaryotic bell-shaped temporal rate of DNA replication origin firing emanates from a balance between origin activation and passivation
dc.typeArticle de revue
dc.identifier.doi10.7554/eLife.35192
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
bordeaux.journaleLife
bordeaux.pagee35192
bordeaux.volume7
bordeaux.peerReviewedoui
hal.identifierhal-01837938
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01837938v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLife&rft.date=2018-06-01&rft.volume=7&rft.spage=e35192&rft.epage=e35192&rft.au=ARBONA,%20Jean-Michel&GOLDAR,%20Arach&HYRIEN,%20Olivier&ARNEODO,%20Alain&AUDIT,%20Benjamin&rft.genre=article


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