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Impact of Growth Conditions on Pseudomonas fluorescens Morphology Characterized by Atomic Force Microscopy

hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierUniversité Badji Mokhtar [Annaba] [UBMA]
dc.contributor.authorKAHLI, Houssem
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGRAUBY-HEYWANG, Christine
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierUniversité Badji Mokhtar [Annaba] [UBMA]
hal.structure.identifierUniversity Chadli Bendjedid El Tarf
dc.contributor.authorDEBEZ, Nesrine
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorGAMMOUDI, Ibtissem
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorMOROTE, F.
hal.structure.identifierUniversité Badji Mokhtar [Annaba] [UBMA]
dc.contributor.authorSBARTAI, Hana
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, Touria
dc.date.issued2022
dc.identifier.issn1661-6596
dc.description.abstractEnThis work is dedicated to the characterization by Atomic Force Microscopy (AFM) of Pseudomonas fluorescens, bacteria having high potential in biotechnology. They were first studied first in optimal conditions in terms of culture medium and temperature. AFM revealed a more-or-less elongated morphology with typical dimensions in the micrometer range, and an organization of the outer membrane characterized by the presence of long and randomly distributed ripples, which are likely related to the organization of lipopolysaccharides (LPS). The outer membrane also presents invaginations, some of them showing a reorganization of ripples, which could be the first sign of a bacterial stress response. In a second step, bacteria grown under unfavorable conditions were characterized. The choice of the medium appeared to be more critical in the case of the second generation of cells, the less adapted medium inducing not only changes in the membrane organization but also larger damages in bacteria. An increased growth temperature affected both the usual "swollen" morphology and the organization of the outer membrane. Here also, LPS likely contribute to membrane remodelling, which makes them potential markers to track cell state changes.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enPseudomonas fluorescens
dc.subject.enGram-negative bacteria
dc.subject.enmorphology
dc.subject.enbacterial surface
dc.subject.enmembrane
dc.subject.enLipopolysaccharide
dc.subject.enstress
dc.title.enImpact of Growth Conditions on Pseudomonas fluorescens Morphology Characterized by Atomic Force Microscopy
dc.typeArticle de revue
dc.identifier.doi10.3390/ijms23179579
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalInternational Journal of Molecular Sciences
bordeaux.page9579
bordeaux.volume23
bordeaux.issue17
bordeaux.peerReviewedoui
hal.identifierhal-03783351
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03783351v1
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