Mostrar el registro sencillo del ítem

Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density

hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorGIANNONE, Gregory
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorHOSY, Eric
hal.structure.identifierPhysiopathologie du système nerveux central - Institut François Magendie
dc.contributor.authorLEVET, Florian
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorCONSTALS, Audrey
hal.structure.identifierInstitute of Biochemistry
dc.contributor.authorSCHULZE, Katrin
hal.structure.identifierVoluum Institute
dc.contributor.authorSOBOLEVSKY, Alexander I.
hal.structure.identifierHoward Hughes Medical Institute [HHMI]
dc.contributor.authorROSCONI, Michael P.
hal.structure.identifierHoward Hughes Medical Institute [HHMI]
dc.contributor.authorGOUAUX, Eric
hal.structure.identifierInstitute of Biochemistry
dc.contributor.authorTAMPÉ, Robert
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorCHOQUET, Daniel
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorCOGNET, Laurent
dc.date.created2010-03-22
dc.date.issued2010-08
dc.identifier.issn0006-3495
dc.description.abstractEnVersatile superresolution imaging methods, able to give dynamic information of endogenous molecules at high density, are still lacking in biological science. Here, superresolved images and diffusion maps of membrane proteins are obtained on living cells. The method consists of recording thousands of single-molecule trajectories that appear sequentially on a cell surface upon continuously labeling molecules of interest. It allows studying any molecules that can be labeled with fluorescent ligands including endogenous membrane proteins on living cells. This approach, named universal PAINT (uPAINT), generalizes the previously developed point-accumulation-for-imaging-in-nanoscale-topography (PAINT) method for dynamic imaging of arbitrary membrane biomolecules. We show here that the unprecedented large statistics obtained by uPAINT on single cells reveal local diffusion properties of specific proteins, either in distinct membrane compartments of adherent cells or in neuronal synapses.
dc.language.isoen
dc.publisherBiophysical Society
dc.title.enDynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bpj.2010.06.005
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.journalBiophysical Journal
bordeaux.page1303-1310
bordeaux.volume99
bordeaux.peerReviewedoui
hal.identifierhal-00661871
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00661871v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biophysical%20Journal&rft.date=2010-08&rft.volume=99&rft.spage=1303-1310&rft.epage=1303-1310&rft.eissn=0006-3495&rft.issn=0006-3495&rft.au=GIANNONE,%20Gregory&HOSY,%20Eric&LEVET,%20Florian&CONSTALS,%20Audrey&SCHULZE,%20Katrin&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem