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Biogeography of Oenococcus oeni reveals distinctive but nonspecific populations in wine-producing regions

hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorEL KHOURY, Mariette
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorCAMPBELL-SILLS, Hugo
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorSALIN, Franck
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorGUICHOUX, Erwan
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCLAISSE, Olivier
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorLUCAS, Patrick M.
dc.date.issued2017
dc.identifier.issn0099-2240
dc.description.abstractEnUnderstanding the mechanisms behind the typicity of regional wines inevitably brings attention to microorganisms associated with their production. Oenococcus oeni is the main bacterial species involved in wine and cider making. It develops after the yeast-driven alcoholic fermentation and performs the malolactic fermentation, which improves the taste and aromatic complexity of most wines. Here, we have evaluated the diversity and specificity of O. oeni strains in six regions. A total of 235 wines and ciders were collected during spontaneous malolactic fermentations and used to isolate 3,212 bacterial colonies. They were typed by multilocus variable analysis, which disclosed a total of 514 O. oeni strains. Their phylogenetic relationships were evaluated by a second typing method based on single nucleotide polymorphism (SNP) analysis. Taken together, the results indicate that each region holds a high diversity of strains that constitute a unique population. However, strains present in each region belong to diverse phylogenetic groups, and the same groups can be detected in different regions, indicating that strains are not genetically adapted to regions. In contrast, greater strain identity was seen for cider, white wine, or red wine of Burgundy, suggesting that genetic adaptation to these products occurred.IMPORTANCE This study reports the isolation, genotyping, and geographic distribution analysis of the largest collection of O. oeni strains performed to date. It reveals that there is very high diversity of strains in each region, the majority of them being detected in a single region. The study also reports the development of an SNP genotyping method that is useful for analyzing the distribution of O. oeni phylogroups. The results show that strains are not genetically adapted to regions but to specific types of wines. They reveal new phylogroups of strains, particularly two phylogroups associated with white wines and red wines of Burgundy. Taken together, the results shed light on the diversity and specificity of wild strains of O. oeni, which is crucial for understanding their real contribution to the unique properties of wines.
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subjectbiogeographie
dc.subjectbactérie lactique
dc.subjectvitis vinifera
dc.subjectoenococcus oeni
dc.subjectoenologie
dc.subjectgénotypage
dc.subjecttypicité du vin
dc.subjectfermentation malolactique
dc.subjectcomposition aromatique
dc.subjectpolymorphisme des nucléotides simples
dc.subject.enbiogeography
dc.subject.enlactic acid bacteria
dc.subject.enwine
dc.subject.enmalolactic fermentation
dc.subject.enSingle nucleotide polymorphism
dc.title.enBiogeography of Oenococcus oeni reveals distinctive but nonspecific populations in wine-producing regions
dc.typeArticle de revue
dc.identifier.doi10.1128/AEM.02322-16
dc.subject.halSciences de l'environnement/Biodiversité et Ecologie
dc.subject.halSciences de l'environnement/Environnement et Société
bordeaux.journalApplied and Environmental Microbiology
bordeaux.page1-11
bordeaux.volume83
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-01606608
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01606608v1
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