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Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorGAUTIER, Florian
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorELIÁŠOVÁ, Kateřina
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEPLÉ, Jean-Charles
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorVONDRAKOVA, Zuzana
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLE METTE, Claire
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
dc.contributor.authorLABEL, Philippe
dc.contributor.authorCOSTA, Guy
hal.structure.identifierPôle Biotechnologie et Sylviculture Avancée
dc.contributor.authorTRONTIN, Jean-François
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorTEYSSIER, Caroline
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLELU-WALTER, Marie-Anne
dc.date.issued2018
dc.identifier.issn1471-2229
dc.description.abstractEnBackground: To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g(-1) f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 (2400 vs 3921 g(-1) f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2ry vs 1ry and 2ry vs 3ry lines at histo-cytological (using LC-MS/MS) and proteomic levels. Results: Repetitive somatic embryogenesis dramatically improved the proliferating lines' cellular organization (genotype SD4's most strongly). Frequencies of singulated, bipolar SEs and compact polyembryogenic centers with elongated suspensors and apparently cleavable embryonal heads increased in 2ry and (even more) 3ry lines. Among 2300-2500 identified proteins, 162 and 228 were classified significantly differentially expressed between 2ry vs 1ry and 3ry vs 2ry lines, respectively, with special emphasis on "Proteolysis" and "Catabolic process" Gene Ontology categories. Strikingly, most of the significant proteins (> 70%) were down-regulated in 2ry relative to 1ry lines, but up-regulated in 3ry relative to 2ry lines, revealing a down-up pattern of expression. GO category enrichment analyses highlighted the opposite adjustments of global protein patterns, particularly for processes involved in chitin catabolism, lignin and L-phenylalanine metabolism, phenylpropanoid biosynthesis, oxidation-reduction, and response to karrikin. Sub-Network Enrichment Analyses highlighted interactions between significant proteins and both plant growth regulators and secondary metabolites after first (especially jasmonic acid, flavonoids) and second (especially salicylic acid, abscisic acid, lignin) embryogenesis cycles. Protein networks established after each induction affected the same "Plant development" and "Defense response" biological processes, but most strongly after the third cycle, which could explain the top embryogenic performance of 3ry lines. Conclusions: This first report of cellular and molecular changes after repetitive somatic embryogenesis in conifers shows that each cycle enhanced the structure and singularization of EMs through modulation of growth regulator pathways, thereby improving the lines' embryogenic status.
dc.language.isoen
dc.publisherBioMed Central
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enDouglas-fir
dc.subject.enEmbryogenic potential
dc.subject.enProliferation
dc.subject.enProteomic
dc.subject.enCytology
dc.subject.enPlant growth regulators
dc.title.enRepetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]
dc.typeArticle de revue
dc.identifier.doi10.1186/s12870-018-1337-y
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalBMC Plant Biology
bordeaux.page24 p.
bordeaux.volume18
bordeaux.peerReviewedoui
hal.identifierhal-02622184
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02622184v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=BMC%20Plant%20Biology&rft.date=2018&rft.volume=18&rft.spage=24%20p.&rft.epage=24%20p.&rft.eissn=1471-2229&rft.issn=1471-2229&rft.au=GAUTIER,%20Florian&ELI%C3%81%C5%A0OV%C3%81,%20Kate%C5%99ina&LEPL%C3%89,%20Jean-Charles&VONDRAKOVA,%20Zuzana&LOMENECH,%20Anne-Marie&rft.genre=article


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