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Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious Mercurialis annua, a Plant with Homomorphic Sex Chromosomes

hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorVELTSOS, Paris
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorCOSSARD, Guillaume
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorBEAUDOING, Emmanuel
hal.structure.identifierCentre National de Ressources Génomiques Végétales [CNRGV]
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorBEYDON, Genséric
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorSAVOVA BIANCHI, Dessislava
hal.structure.identifierÉvolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorROUX, Camille
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorC. GONZÁLEZ-MARTÍNEZ, Santiago
hal.structure.identifierDepartment of Ecology and Evolution [UNIL, Lausanne] = Département d'écologie et évolution [DEE]
dc.contributor.authorR. PANNELL, John
dc.date.issued2018-06
dc.identifier.issn2073-4425
dc.description.abstractEnDioecious plants vary in whether their sex chromosomes are heteromorphic or homomorphic, but even homomorphic sex chromosomes may show divergence between homologues in the non-recombining, sex-determining region (SDR). Very little is known about the SDR of these species, which might represent particularly early stages of sex-chromosome evolution. Here, we assess the size and content of the SDR of the diploid dioecious herb Mercurialis annua, a species with homomorphic sex chromosomes and mild Y-chromosome degeneration. We used RNA sequencing (RNAseq) to identify new Y-linked markers for M. annua. Twelve of 24 transcripts showing male-specific expression in a previous experiment could be amplified by polymerase chain reaction (PCR) only from males, and are thus likely to be Y-linked. Analysis of genome-capture data from multiple populations of M. annua pointed to an additional six male-limited (and thus Y-linked) sequences. We used these markers to identify and sequence 17 sex-linked bacterial artificial chromosomes (BACs), which form 11 groups of non-overlapping sequences, covering a total sequence length of about 1.5 Mb. Content analysis of this region suggests that it is enriched for repeats, has low gene density, and contains few candidate sex-determining genes. The BACs map to a subset of the sex-linked region of the genetic map, which we estimate to be at least 14.5 Mb. This is substantially larger than estimates for other dioecious plants with homomorphic sex chromosomes, both in absolute terms and relative to their genome sizes. Our data provide a rare, high-resolution view of the homomorphic Y chromosome of a dioecious plant.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.engenetic map
dc.subject.entransposable element
dc.subject.enRNA sequencing (RNAseq)
dc.subject.enbacterial artificial chromosomes (BAC)
dc.subject.engene density
dc.title.enSize and Content of the Sex-Determining Region of the Y Chromosome in Dioecious Mercurialis annua, a Plant with Homomorphic Sex Chromosomes
dc.typeArticle de revue
dc.identifier.doi10.3390/genes9060277
dc.subject.halSciences du Vivant [q-bio]/Génétique/Génétique des populations [q-bio.PE]
bordeaux.journalGenes
bordeaux.page277
bordeaux.volume9
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-01806943
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01806943v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genes&rft.date=2018-06&rft.volume=9&rft.issue=6&rft.spage=277&rft.epage=277&rft.eissn=2073-4425&rft.issn=2073-4425&rft.au=VELTSOS,%20Paris&COSSARD,%20Guillaume&BEAUDOING,%20Emmanuel&BEYDON,%20Gens%C3%A9ric&SAVOVA%20BIANCHI,%20Dessislava&rft.genre=article


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