X-ray microtomography (micro-CT): a reference technology for high-resolution quantification of xylem embolism in trees
COCHARD, Hervé
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
BADEL, Eric
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
COCHARD, Hervé
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
BADEL, Eric
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
< Leer menos
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
Idioma
en
Article de revue
Este ítem está publicado en
Plant, Cell and Environment. 2015, vol. 38, n° 1, p. 201-206
Wiley
Resumen en inglés
As current methods for measuring xylem embolism in trees are indirect and prone to artefacts, there is an ongoing controversy over the capacity of trees to resist or recover from embolism. The debate will not end until we ...Leer más >
As current methods for measuring xylem embolism in trees are indirect and prone to artefacts, there is an ongoing controversy over the capacity of trees to resist or recover from embolism. The debate will not end until we get direct visualization of the vessel content. Here, we propose desktop X-ray microtomography (micro-CT) as a reference direct technique to quantify xylem embolism and thus validate more widespread measurements based upon either hydraulic or acoustic methods. We used desktop micro-CT to measure embolism levels in dehydrated or centrifuged shoots of laurel – a long-vesseled species thought to display daily cycles of embolism formation and refilling. Our direct observations demonstrate that this Mediterranean species is highly resistant to embolism and is not vulnerable to drought-induced embolism in a normal range of xylem tensions. We therefore recommend that embolism studies in long-vesseled species should be validated by direct methods such as micro-CT to clear up any misunderstandings on their physiology.< Leer menos
Palabras clave en inglés
cavitation
imaging
methods
xylem physiology
Orígen
Importado de HalCentros de investigación