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Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level

hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorALBERTIN, Warren
hal.structure.identifierInstitut des Sciences de la Vigne et du Vin (ISVV)
dc.contributor.authorPANFILI, Aurélie
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMIOT-SERTIER, Cecile
hal.structure.identifierInstitut des Sciences de la Vigne et du Vin (ISVV)
dc.contributor.authorGOULIELMAKIS, Aurélie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDELCAMP, Adline
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorSALIN, Franck
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorLONVAUD-FUNEL, Aline
hal.structure.identifierAustralian Wine Research Institute [AWRI]
dc.contributor.authorCURTIN, Chris
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMASNEUF-POMAREDE, Isabelle
dc.date.issued2014
dc.identifier.issn0740-0020
dc.description.abstractEnAlthough many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.
dc.language.isoen
dc.publisherElsevier
dc.title.enDevelopment of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level
dc.typeArticle de revue
dc.identifier.doi10.1016/j.fm.2014.03.012
dc.subject.halSciences du Vivant [q-bio]/Ingénierie des aliments
dc.subject.halSciences de l'ingénieur [physics]/Génie des procédés
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalFood Microbiology
bordeaux.page188–195
bordeaux.volume42
bordeaux.issueSeptembre
bordeaux.peerReviewedoui
hal.identifierhal-02636791
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02636791v1
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