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Quantitative proteomic and phosphoproteomic approaches for deciphering the signaling pathway for tension wood formation in poplar

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMAURIAT, Mélanie
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorLEPLÉ, Jean-Charles
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBARTHOLOMÉ, Jérôme
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorNÉGRONI, Luc
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorRICHET, Nicolas
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBOONNEU, Marc
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
dc.contributor.authorCOUTAND, Catherine
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.issued2015
dc.identifier.issn1535-3893
dc.description.abstractEnTrees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3 763 nonphosphorylated peptides, corresponding to 1 155 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectphosphoproteomics
dc.subjectproteomics
dc.subjectpoplar
dc.subject.enwood formation
dc.subject.entension wood
dc.subject.encell wall
dc.title.enQuantitative proteomic and phosphoproteomic approaches for deciphering the signaling pathway for tension wood formation in poplar
dc.typeArticle de revue
dc.identifier.doi10.1021/acs.jproteome.5b00140
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
bordeaux.journalJournal of Proteome Research
bordeaux.page3188-3203
bordeaux.volume14
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-02640086
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640086v1
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