De novo assembly of maritime pine transcriptome: implications for forest breeding and biotechnology
LABEL, Philippe
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
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Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
LABEL, Philippe
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier [PIAF]
GUEVARA, María-Angeles
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology [INIA]
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology [INIA]
RODRIGUES, Andreia
Instituto de Biologia Experimental e Tecnológica [IBET]
Instituto de Technologia Quimica e Biologica
Instituto de Biologia Experimental e Tecnológica [IBET]
Instituto de Technologia Quimica e Biologica
LELU-WALTER, Marie-Anne
Unité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
Unité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
MIGUEL, Célia
Instituto de Biologia Experimental e Tecnológica [IBET]
Instituto de Technologia Quimica e Biologica
Instituto de Biologia Experimental e Tecnológica [IBET]
Instituto de Technologia Quimica e Biologica
CERVERA, María Teresa
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology [INIA]
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology [INIA]
Language
en
Article de revue
This item was published in
Plant Biotechnology Journal. 2014, vol. 12, n° 3, p. 286-299
Wiley
English Abstract
Maritime pine (Pinus pinasterAit.) is a widely distributed conifer species in Southwestern Europe and one of the most advanced models for conifer research. In the current work, comprehensive characterization of the maritime ...Read more >
Maritime pine (Pinus pinasterAit.) is a widely distributed conifer species in Southwestern Europe and one of the most advanced models for conifer research. In the current work, comprehensive characterization of the maritime pine transcriptome was performed using a combination of two different next-generation sequencing platforms, 454 and Illumina. De novo assembly of the transcriptome provided a catalogue of 26 020 unique transcripts in maritime pine trees and a collection of 9641 full-length cDNAs. Quality of the transcriptome assembly was validated by RT-PCR amplification of selected transcripts for structural and regulatory genes. Transcription factors and enzyme-encoding transcripts were annotated. Furthermore, the available sequencing data permitted the identification of polymorphisms and the establishment of robust single nucleotide polymorphism (SNP) and simple-sequence repeat (SSR) databases for genotyping applications and integration of translational genomics in maritime pine breeding programmes. All our data are freely available at SustainpineDB, the P. pinaster expressional database. Results reported here on the maritime pine transcriptome represent a valuable resource for future basic and applied studies on this ecological and economically important pine species.Read less <
Keywords
transcription factors
assemblage du transcriptome
English Keywords
conifers
full-length cDNA
next-generation sequencing
single nucleotide polymorphism
transcriptome sequencing
Origin
Hal imported