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Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen

hal.structure.identifierDepartment of Wood Science
dc.contributor.authorDIGUISTINI, Scott
hal.structure.identifierDepartment of Wood Science
dc.contributor.authorWANG, Ye
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorLIAO, Nancy Y.
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorTAYLOR, Greg
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorTANGUAY, Philippe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorFEAU, Nicolas
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
dc.contributor.authorHENRISSAT, Bernard
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorCHAN, Simon K.
hal.structure.identifierDepartment of Wood Science
dc.contributor.authorHESSE-ORCE, Uljana
hal.structure.identifierDepartment of Wood Science
dc.contributor.authorMASSOUMI ALAMOUTI, Sepideh
hal.structure.identifierUniversity of British Columbia [UBC]
dc.contributor.authorTSUI, Clement K. M.
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorDOCKING, Roderick T.
hal.structure.identifierUnité mixte de recherche de biotechnologie des champignons filamenteux
dc.contributor.authorLEVASSEUR, Anthony
hal.structure.identifierDepartment of Wood Science
dc.contributor.authorHARIDAS, Sajeet
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorROBERTSON, Gordon
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorBIROL, Inanç
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorHOLT, Robert A.
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorMARRA, Marco A.
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorHAMELIN, Richard C.
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorHIRST, Martin
hal.structure.identifierBritish Columbia Cancer Agency
dc.contributor.authorJONES, Steven J.M.
hal.structure.identifierUniversity of British Columbia [UBC]
dc.contributor.authorBOHLMANN, Jörg
hal.structure.identifierDepartment of Wood Science
dc.contributor.authorBREUIL, Colette
dc.date.issued2011
dc.identifier.issn0027-8424
dc.description.abstractEnIn western North America, the current outbreak of the mountain pine beetle (MPB) and its microbial associates has destroyed wide areas of lodgepole pine forest, including more than 16 million hectares in British Columbia. Grosmannia clavigera (Gc), a critical component of the outbreak, is a symbiont of the MPB and a pathogen of pine trees. To better understand the interactions between Gc, MPB, and lodgepole pine hosts, we sequenced the ∼30-Mb Gc genome and assembled it into 18 supercontigs. We predict 8,314 protein-coding genes, and support the gene models with proteome, expressed sequence tag, and RNA-seq data. We establish that Gc is heterothallic, and report evidence for repeat-induced point mutation. We report insights, from genome and transcriptome analyses, into how Gc tolerates conifer-defense chemicals, including oleoresin terpenoids, as they colonize a host tree. RNA-seq data indicate that terpenoids induce a substantial antimicrobial stress in Gc, and suggest that the fungus may detoxify these chemicals by using them as a carbon source. Terpenoid treatment strongly activated a ∼100-kb region of the Gc genome that contains a set of genes that may be important for detoxification of these host-defense chemicals. This work is a major step toward understanding the biological interactions between the tripartite MPB/fungus/forest system.
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.subjectABC TRANSPORTER
dc.subjectFOREST GENOMICS
dc.subjectGENOMIQUE
dc.subjectDENDROCTONE DU PIN
dc.subjectLUTTE CONTRE LES MALADIES
dc.subject.enGROSMANNIA CLAVIGERA
dc.subject.enNEXT GENERATION SEQUENCING
dc.subject.enMONOTERPENE
dc.subject.enCARBOHYDRATE ACTIVE ENZYMES
dc.title.enGenome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen
dc.typeArticle de revue
dc.identifier.doi10.1073/pnas.1011289108
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
bordeaux.journalProceedings of the National Academy of Sciences of the United States of America
bordeaux.page2504-2509
bordeaux.volume108
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02644831
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02644831v1
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