VORNAM, B.; GAILING, O.; DERORY, Jérémy; PLOMION, Christophe; KREMER, Antoine; FINKELDEY, R.

doi:10.1111/j.1438-8677.2011.00446.x

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VORNAM, B.
Georg-August-University = Georg-August-Universität Göttingen

GAILING, O.
Georg-August-University = Georg-August-Universität Göttingen
Michigan Technological University [MTU]

DERORY, Jérémy
Biodiversité, Gènes & Communautés [BioGeCo]

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Plant Biology. 2011, vol. 13, n° 6, p. 881 - 887

Wiley

Resumen en inglés

For the first time in sessile oak [Quercus petraea (Matt.) Liebl.], the isolation and characterisation of a full-length dehydrin gene and its promoter region, as well as its allelic variation in natural populations, is reported. Dehydrins (Dhn) are stress-related genes important for the survival of perennial plants in a seasonal climate. A full-length dehydrin gene (Dhn3) was characterised at the nucleotide level and the protein structure was modelled. Additionally, the allelic variation was analysed in five natural populations of Quercus petraea (Matt.) Liebl. sampled along an altitudinal gradient in the French Pyrenees. The analysed sequences contain typical domains of the K(n) class of dehydrins in the coding region. Also, the 5'untranslated region (promoter) of the gene was amplified, which shows typical motifs essential for drought- and cold-responsive gene expression. Single nucleotide substitutions and indels (insertions/deletions) within the coding region determine large biochemical differences at the protein level. However, only low levels of genetic differentiation between populations from different altitudes were detectable.< Leer menos

Palabras clave

plant sciences

quercus petraea

quercus sessiliflora

chene rouvre

génétique des populations

chene sessile

Palabras clave en inglés

dehydrin

drought

sessile oak

SNPs

cold