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Interaction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.)

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBEDON, Franck
hal.structure.identifierSERIDA, Forestry Section
dc.contributor.authorMAJADA, Juan
hal.structure.identifierSERIDA, Forestry Section
dc.contributor.authorFEITO, Isabel
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorDUPUY, Jean-William
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierCentre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
dc.contributor.authorGION, Jean-Marc
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.issued2011
dc.identifier.issn0981-9428
dc.description.abstractEnEucalyptus globulus (Labill.) is used for pulp and paper production worldwide. In this report we studied changes in protein expression in one osmotically stressed elite clone widely used in industrial plantations in Spain. High molecular weight polyethylene glycol (PEG) was used as an osmoticum in the growing medium. Roots of rooted cuttings were sampled after 3 and 36 h of treatment. Water potential and abscissic acid content were measured in shoot and root apices to characterize the physiological states of the plants. Total soluble proteins from roots were extracted and separated using two-dimensional gel electrophoresis (2-DE). Gels were stained with Coomassie brillant blue for quantitative analysis of protein accumulation. From a total of 406 reproducible spots, 34 were found to be differentially expressed depending on treatment (osmotic versus control condition) and/or stress duration (3 h versus 36 h), and were further characterized by tandem mass spectrometry. Several proteins were reliably identified including adenosine kinase, actin, stress-related proteins as well as proteins associated to cellular processes, among which some residents of the endoplasmic reticulum. This study constitutes the first investigation of the root proteome in this important forest tree genus.
dc.language.isoen
dc.publisherElsevier
dc.subjectEUCALYPTUS GLOBULUS
dc.subject2-DE
dc.subject.enROOT
dc.subject.enPROTEOME
dc.subject.enOSMOTIC STRESS
dc.subject.enWATER POTENTIAL
dc.subject.enENDOPLASMIC RETICULUM
dc.title.enInteraction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.)
dc.typeArticle de revue
dc.identifier.doi10.1016/j.plaphy.2010.09.020
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPlant Physiology and Biochemistry
bordeaux.page69-76
bordeaux.volume49
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02647579
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647579v1
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