Afficher la notice abrégée

Challenges of microsatellite isolation in fungi

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDUTECH, Christian Cyril
hal.structure.identifierBIOlogie et GEstion des Risques en agriculture [BIOGER]
dc.contributor.authorENJALBERT, Jerome
hal.structure.identifierBiologie et Génétique des Interactions Plantes-Agents Pathogènes
dc.contributor.authorFOURNIER, Elisabeth
hal.structure.identifierUnité Mixte de Recherche en Santé Végétale (INRA/ENITA) [UMRSV]
dc.contributor.authorDELMOTTE, François
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorBARRES, Benoît
hal.structure.identifierBiologie et Génétique des Interactions Plantes-Agents Pathogènes
dc.contributor.authorCARLIER, Jean
hal.structure.identifierBiologie et Génétique des Interactions Plantes-Agents Pathogènes
dc.contributor.authorTHARREAU, Didier
hal.structure.identifierEcologie Systématique et Evolution [ESE]
hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorGIRAUD, Tatiana
dc.date.issued2007
dc.identifier.issn1087-1845
dc.description.abstractEnAlthough they represent powerful genetic markers in many fields of biology, microsatellites have been isolated in few fungal species. The aim of this study was to assess whether obtaining microsatellite markers with an acceptable level of polymorphism is generally harder from fungi than in other organisms. We therefore surveyed the number,nature and polymorphism level of published microsatellite markers in fungi from the literature and from our own data on seventeen fungal microsatellite-enriched libraries, and in five other phylogroups (angiosperms, insects, fishes, birds and mammals). Fungal microsatellites indeed appeared both harder to isolate and to exhibit lower polymorphism than in other organisms. This appeared to be due, at least in part, to genomic specificities, such as scarcity and shortness of fungal microsatellite loci. A correlation was observed between mean repeat number and mean allele number in the published fungal microsatellite loci. The cross-species transferability of fungal microsatellites also appeared lower than in other phylogroups. However, microsatellites have been useful in some fungal species. Thus, the considerable advantages of these markers make their development worthwhile, and this study provides some guidelines for their isolation.
dc.language.isoen
dc.publisherElsevier
dc.subject.enSSR
dc.subject.enPLANT PATHOGEN
dc.subject.enPOLYMORPHIC
dc.subject.enMARKERS
dc.subject.enDEVELOPMENT
dc.subject.enISOLATION
dc.subject.enFEATURES
dc.subject.enAFFECTING POLYMORPHISM
dc.title.enChallenges of microsatellite isolation in fungi
dc.typeArticle de revue
dc.identifier.doi10.1016/j.fgb.2007.05.003
dc.subject.halSciences du Vivant [q-bio]/Génétique
bordeaux.journalFungal Genetics and Biology
bordeaux.page933-949
bordeaux.volume44
bordeaux.issue10
bordeaux.peerReviewednon
hal.identifierhal-02667556
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02667556v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Fungal%20Genetics%20and%20Biology&rft.date=2007&rft.volume=44&rft.issue=10&rft.spage=933-949&rft.epage=933-949&rft.eissn=1087-1845&rft.issn=1087-1845&rft.au=DUTECH,%20Christian%20Cyril&ENJALBERT,%20Jerome&FOURNIER,%20Elisabeth&DELMOTTE,%20Fran%C3%A7ois&BARRES,%20Beno%C3%AEt&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée