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The proteome of maritime pine wood forming tissue

hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorGION, Jean-Marc
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorLE PROVOST, Grégoire
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorFERRY-DUMAZET, Hélène
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
hal.structure.identifierInstituto de Technologia Quimica e Biologica
dc.contributor.authorPAIVA, Jorge Almiro
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorFRIGERIO, Jean-Marc
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorBRACH, Jean
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorDE DARUVAR, Antoine
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBONNEU, Marc
hal.structure.identifierUnité de Recherche Protéomique [PROTEOMIQUE]
dc.contributor.authorSOMMERER, Nicolas
hal.structure.identifierGénétique Végétale [GV]
dc.contributor.authorNÉGRONI, Luc
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.issued2005
dc.identifier.issn1615-9853
dc.description.abstractEnWood is one of our most important natural resources. Surprisingly, we know hardly anything about the details of the process of wood formation. The aim of this work was to describe the main proteins expressed in wood forming tissue of a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 to 7, a total of 1039 spots were detected. Out of the 240 spots analyzed by MS/MS, 67.9% were identified, 16.7% presented no homology in the databases, and 15.4% corresponded to protein mixtures. Out of the 57 spots analyzed by MALDI-MS, only 15.8% were identified. Most of the 175 identified proteins play a role in either defense (19.4%), carbohydrates (16.6%) and amino acid (14.9%) metabolisms, genes and proteins expression (13.1%), cytoskeleton (8%), cell wall biosynthesis (5.7%), secondary (5.1%) and primary (4%) metabolisms. A summary of the identified proteins, their putative functions, and behavior in different types of wood are presented. This information was introduced into the PROTICdb database and is accessible at http://cbib1.cbib.u-bordeaux2.fr/Protic/Protic/home/index.php. Finally, the average protein amount was compared with their respective transcript abundance as quantified through EST counting in a cDNA-library constructed with mRNA extracted from wood forming tissue
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subjectPINUS PINASTER AIT.
dc.subject.enMASS SPECTROMETRY
dc.subject.enPROTEOME ANALYSIS
dc.subject.enWOOD
dc.title.enThe proteome of maritime pine wood forming tissue
dc.typeArticle de revue
dc.identifier.doi10.1002/pmic.200401197
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalProteomics
bordeaux.page3731-3751
bordeaux.volume5
bordeaux.issue14
bordeaux.peerReviewedoui
hal.identifierhal-02678286
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02678286v1
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