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Coupling evolutionary dynamics of Venturia inaequalis effectors and functional genomic to decipher mechanisms of virulence and to identify durable resistance genes in apple.

hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorCHEVREAU, Elisabeth
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorCALMES, Benoit
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEROY, Thibaut
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorBIESSY, Adrien
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorGUILLEMETTE, Thomas
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorSANNIER, Mélanie
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorEXPERT, Pascale
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorDE GRACIA, Marie
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorCHARRIER, Aurelie
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorCOLLEMARE, Jérôme
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorCAFFIER, Valérie
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorVERGNE-GAILLARD, Emilie
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorDUREL, Charles Eric
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorLEMAIRE, Christophe
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorLE CAM, Bruno
dc.date.issued2015
dc.date.conference2015-03-17
dc.description.abstractEnDuring infection, pathogens secrete small secreted proteins (SSPs), called effectors, that promote disease. Plant receptors encoded by resistance R genes might recognize such effectors (also called avirulence factors AVRs), resulting in plant immunity. Pathogens evade recognition thanks to the emergence of virulent alleles present in populations. It has been demonstrated that avirulent effectors are crucial for the pathogen infection cycle and that their loss-of-function may induce a substantial fitness cost. This kind of effector is expected to be under purifying selective pressure. Here, we aim at identifying the effector repertoire of Venturia inaequalis, the agent of apple scab, assessing its evolutionary dynamics and studying the role of candidate effectors in virulence. We sequenced de novo 90 strains, collected on apple and on their wild relatives and differing in their host range or virulence to study allelic polymorphism at 880 putative effector loci. The top-20 hits for highly conserved sequences were selected as candidates for further functional analyses. In planta gene expression showed a significant induction of these conserved SSP at the early stage of plant infection. Their functions were investigated using targeted deletion mutants. Remarkably, loss of two conserved SSPs resulted in reduced aggressiveness without any alteration in growth in vitro. GFP-tagged protein and heterologous expression were used to assess their sub-cellular localization in infected apple leaves. Involvement of theses SSP in the modulation of host defence was also investigated using an apple full-transcript microarray. Highly conserved effectors will be used to screen for novel R genes in Malus genotypes characterized for their high resistance to scab. This combined knowledge should enable us to understand strategies used by the pathogen to overcome defences in apple and consequently to build more durable resistance towards apple scab.
dc.language.isoen
dc.subjectpopulation
dc.subject.eneffector
dc.subject.entranscription profiling
dc.title.enCoupling evolutionary dynamics of Venturia inaequalis effectors and functional genomic to decipher mechanisms of virulence and to identify durable resistance genes in apple.
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences de l'environnement
bordeaux.countryUS
bordeaux.conference.cityPacific Grove
bordeaux.peerReviewedoui
hal.identifierhal-02799357
hal.version1
hal.invitednon
hal.conference.end2015-03-22
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02799357v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2015&rft.au=CHEVREAU,%20Elisabeth&CALMES,%20Benoit&LEROY,%20Thibaut&BIESSY,%20Adrien&GUILLEMETTE,%20Thomas&rft.genre=unknown


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