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A proteomic study of water deficit-responsive proteins in poplar roots

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVINCENT, Delphine
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMARCO, Julie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBONNEU, Marc
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierEcologie et Ecophysiologie Forestières [devient SILVA en 2018] [EEF]
dc.contributor.authorBOGEAT-TRIBOULOT, Marie-Béatrice
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.accessioned2022-10-12T12:25:52Z
dc.date.available2022-10-12T12:25:52Z
dc.date.issued2009
dc.date.conference2009-01-09
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/156211
dc.description.abstractEnIn order to analyze root proteins involved in drought tolerance in poplar, a proteomic study was conducted on the roots of two Populus deltoïdes × Populus nigra cultivars, Carpaccio and Soligo. These cultivars were selected based on contrasted physiological responses under water stress. Plants were exposed to different water stress levels (Control, Early, Medium and Severe), and root tips collected. Technical optimization included protein extraction and resolubilization, as well as the finetuning of electrophoretic conditions. The best results were obtained upon using phenol extraction, a resolubilization solution containing two reduction reagents, DTT and 2-ME, and 100 μg protein load on both acidic (pH 4-7) and basic (pH 7-11NL) IPG strips. A total of 48 2-D gels were then produced and quantitatively analyzed using SameSpot Progenesis software. Statistical analyses were completed using normalized spot volumes. Multivariate analyses (correlation matrix, PCA and HCA) and twoway ANOVAs showed that protein expression was first regulated by genotype effect and second by treatment effect. Differentially regulated proteins are being identified based on tandem mass spectrometry. This functional information should shed some lights on the molecular players involved in drought-stress response and potentially underlying adaptation to this abiotic constraint.
dc.language.isoen
dc.subjectPOPLAR
dc.subject.enWATER DEFICIT
dc.subject.enDROUGHT TOLERANCE
dc.subject.enPROTEOMIC
dc.title.enA proteomic study of water deficit-responsive proteins in poplar roots
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]
bordeaux.pagen.p.
bordeaux.hal.laboratoriesBioGeCo (Biodiversité Gènes & Communautés) - UMR 1202*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.countryUS
bordeaux.title.proceeding17. Genomes Conference. Plant, Animal and Microbe
bordeaux.conference.citySan Diego
bordeaux.peerReviewedoui
hal.identifierhal-02819720
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02819720v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2009&rft.spage=n.p.&rft.epage=n.p.&rft.au=VINCENT,%20Delphine&MARCO,%20Julie&CHAUMEIL,%20Philippe&BONNEU,%20Marc&CLAVEROL,%20St%C3%A9phane&rft.genre=conference


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