Protein Extraction from Woody Plants
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| dc.contributor.author | PLOMION, Christophe | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| dc.contributor.author | LALANNE, Céline | |
| dc.date.accessioned | 2022-10-12T12:25:19Z | |
| dc.date.available | 2022-10-12T12:25:19Z | |
| dc.date.issued | 2007 | |
| dc.identifier.isbn | 978-1-58829-635-1 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/156155 | |
| dc.description.abstractEn | In this chapter we present a protocol for total protein extraction optimized for woodforming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php. | |
| dc.language.iso | en | |
| dc.publisher | Humana Press | |
| dc.publisher.location | Totawa (united states) | |
| dc.source.title | Plant Proteomics: Methods and Protocols | |
| dc.subject | TOTAL PROTEIN EXTRACTION | |
| dc.subject.en | TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS | |
| dc.subject.en | WOOD | |
| dc.subject.en | TREE | |
| dc.subject.en | ESPECE A FEUILLES LARGES | |
| dc.subject.en | METHODE TCA-ACETONE | |
| dc.subject.en | ELECTROPHORESE EN GEL DE POLYACRYLAMIDE | |
| dc.title.en | Protein Extraction from Woody Plants | |
| dc.type | Chapitre d'ouvrage | |
| dc.identifier.doi | 10.1385/1-59745-227-0:37 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| bordeaux.volume | 355 | |
| bordeaux.hal.laboratories | BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | INRAE | |
| hal.identifier | hal-02823006 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02823006v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Plant%20Proteomics:%20Methods%20and%20Protocols&rft.date=2007&rft.volume=355&rft.au=PLOMION,%20Christophe&LALANNE,%20C%C3%A9line&rft.isbn=978-1-58829-635-1&rft.genre=unknown |
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