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High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries

hal.structure.identifierInteractions Biotiques et Santé Végétale [IBSV]
dc.contributor.authorMALAUSA, Thibaut
hal.structure.identifierAix Marseille Université [AMU]
dc.contributor.authorGILLES, André
hal.structure.identifierAix Marseille Université [AMU]
dc.contributor.authorMEGLÉCZ, Emese
hal.structure.identifierGenoscreen
dc.contributor.authorBLANCARD, Hélène
hal.structure.identifierGenoscreen
dc.contributor.authorDUTHOY, Stéphanie
hal.structure.identifierAix Marseille Université [AMU]
dc.contributor.authorCOSTEDOAT, Caroline
hal.structure.identifierAix Marseille Université [AMU]
dc.contributor.authorDUBUT, Vincent
hal.structure.identifierAix Marseille Université [AMU]
dc.contributor.authorPECH, Nicolas
hal.structure.identifierInteractions Biotiques et Santé Végétale [IBSV]
dc.contributor.authorCASTAGNONE-SERENO, Philippe
hal.structure.identifierInteractions Biotiques et Santé Végétale [IBSV]
dc.contributor.authorDELYE, Christophe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorFEAU, Nicolas
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorFREY, Pascal
hal.structure.identifierCentre de Biologie pour la Gestion des Populations [UMR CBGP]
dc.contributor.authorGAUTHIER, Philippe
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorGUILLEMAUD, Thomas
hal.structure.identifierAGroécologie, Innovations, teRritoires [AGIR]
dc.contributor.authorHAZARD, Laurent
hal.structure.identifierFLAveur, VIsion et Comportement du consommateur [FLAVIC]
dc.contributor.authorLE CORRE, Valérie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLUNG, Brigitte
hal.structure.identifierCentre de Biologie pour la Gestion des Populations [UMR CBGP]
dc.contributor.authorMALE, Pierre-Jean
hal.structure.identifierGenoscreen
dc.contributor.authorFERREIRA, Stéphanie
hal.structure.identifierCentre de Biologie pour la Gestion des Populations [UMR CBGP]
dc.contributor.authorMARTIN, Jean-François
dc.date.issued2011
dc.identifier.issn1755-098X
dc.description.abstractEnMicrosatellites (or SSRs: simple sequence repeats) are among the most frequently used DNA markers in many areas of research. The use of microsatellite markers is limited by the difficulties involved in their de novo isolation from species for which no genomic resources are available. We describe here a high-throughput method for isolating microsatellite markers based on coupling multiplex microsatellite enrichment and next-generation sequencing on 454 GS-FLX Titanium platforms. The procedure was calibrated on a model species (Apis mellifera) and validated on 13 other species from various taxonomic groups (animals, plants and fungi), including taxa for which severe difficulties were previously encountered using traditional methods. We obtained from 11 497 to 34 483 sequences depending on the species and the number of detected microsatellite loci ranged from 199 to 5791. We thus demonstrated that this procedure can be readily and successfully applied to a large variety of taxonomic groups, at much lower cost than would have been possible with traditional protocols. This method is expected to speed up the acquisition of high-quality genetic markers for nonmodel organisms.
dc.language.isoen
dc.publisherWiley/Blackwell
dc.subjectmarqueur microsatellite
dc.subjectgénotypage
dc.subjectpyroséquençage
dc.subjectbanque d'adn
dc.subjecttaxonomie
dc.subjectpcr
dc.subjectmarqueur génétique
dc.subject.enmicrosatellite repeats
dc.subject.enprimer design
dc.subject.enpyrosequencing
dc.subject.enenriched library
dc.subject.engenetic marker
dc.subject.engenotyping
dc.subject.enmicrosatellite isolation
dc.subject.ennext-generation sequencing
dc.subject.entaxonomy
dc.title.enHigh-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1755-0998.2011.02992.x
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalMolecular Ecology Resources
bordeaux.page638-644
bordeaux.volume11
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-01506135
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01506135v1
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