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Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

hal.structure.identifierUnité de Mycologie [USC INRAE LSV-mycologie]
dc.contributor.authorAGUAYO, Jaime
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
hal.structure.identifierMinistère de l'Alimentation, de l'Agriculture et de la Pêche [Paris, France]
dc.contributor.authorHUSSON, Claude
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHANCEREL, Emilie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorFABREGUETTES, Olivier
hal.structure.identifierCentre Wallon de Recherches Agronomiques [CRA-W]
dc.contributor.authorCHANDELIER, Anne
hal.structure.identifierUnité de Mycologie [USC INRAE LSV-mycologie]
dc.contributor.authorFOURRIER‐JEANDEL, Céline
hal.structure.identifierRéseau National de Surveillance Aérobiologique [RNSA]
dc.contributor.authorDUPUY, Nadine
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDUTECH, Cyril
hal.structure.identifierUnité de Mycologie [USC INRAE LSV-mycologie]
dc.contributor.authorIOOS, Renaud
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorROBIN, Cécile
hal.structure.identifierRéseau National de Surveillance Aérobiologique [RNSA]
dc.contributor.authorTHIBAUDON, Michel
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorMARÇAIS, Benoit
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDESPREZ‐LOUSTAU, Marie‐laure
dc.date.issued2021
dc.identifier.issn0032-0862
dc.description.abstractEnForest disease management relies principally on a preventive approach in which epidemiological surveillance plays a crucial role. However, efficient and cost‐effective surveillance methods are not currently available for large spatial scales. Nevertheless, aerobiological networks have been set up for several decades in many countries to monitor pollen dispersal and provide real‐time assessments of allergenic risk. Here, we suggest that the same approach could be used for the surveillance of forest pathogens. Using molecular methods, we analysed samples from 12 sites of the French aerobiological network, at different dates. Both metabarcoding by high‐throughput sequencing (using two markers and two different bioinformatics approaches) and real‐time PCR targeting eight important forest pathogens were conducted. To validate the approach, temporal and spatial trends of spore detection were compared with field disease data. The metabarcoding approach demonstrated that many fungal plant pathogens could be found in aerobiological samples. Moreover, five of the eight targeted forest pathogens were detected by real‐time PCR, with temporal and spatial trends of spore capture consistent with field data. In particular, Hymenoscyphus fraxineus was detected at high frequency in aerobiological samples in the areas where ash dieback has been present for the longest period of time, and at lower frequency in areas with more recent invasion. Spore detection of seasonal pathogens showed a temporal pattern similar to that of disease reports. Overall, our study provides a proof of concept that permanent aerobiological networks combined with molecular methods may provide a useful tool for large‐scale surveillance of forest pathogens.
dc.description.sponsorshipRecherches Avancées sur l'Arbre et les Ecosytèmes Forestiers - ANR-11-LABX-0002
dc.language.isoen
dc.publisherWiley
dc.subject.enplant disease surveillance
dc.subject.enAerobiology
dc.subject.enHymenoscyphus fraxineus
dc.subject.enMetabarcoding
dc.subject.enPlant disease surveillance
dc.subject.enSpore dispersal
dc.subject.enSpore trap
dc.subject.enspore dispersal
dc.subject.enspore trap
dc.subject.enmetabarcoding
dc.subject.meshPlant Diseases
dc.subject.meshForests
dc.title.enCombining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept
dc.typeArticle de revue
dc.identifier.doi10.1111/ppa.13265
dc.subject.halSciences de l'environnement
dc.description.sponsorshipEuropeHOlistic Management of Emerging forest pests and Diseases,
bordeaux.journalPlant Pathology
bordeaux.page181-194
bordeaux.volume70
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02950504
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02950504v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Pathology&rft.date=2021&rft.volume=70&rft.issue=1&rft.spage=181-194&rft.epage=181-194&rft.eissn=0032-0862&rft.issn=0032-0862&rft.au=AGUAYO,%20Jaime&HUSSON,%20Claude&CHANCEREL,%20Emilie&FABREGUETTES,%20Olivier&CHANDELIER,%20Anne&rft.genre=article


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