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Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii

hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorTEYSSIER, Caroline
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
hal.structure.identifierUniversité de Limoges [UNILIM]
dc.contributor.authorGAUTIER, Florian
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorELIÁŠOVÁ, Kateřina
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEPLÉ, Jean-Charles
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorVONDRAKOVA, Zuzana
hal.structure.identifierCentre Génomique Fonctionnelle Bordeaux [Bordeaux] [CGFB]
dc.contributor.authorLOMENECH, Anne Marie
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLE METTE, Claire
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
dc.contributor.authorLABEL, Philippe
hal.structure.identifierUniversité de Limoges [UNILIM]
dc.contributor.authorCOSTA, Guy
hal.structure.identifierInstitut Technologique Forêt Cellulose Bois-construction Ameublement [FCBA]
dc.contributor.authorTRONTIN, Jean-François
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLELU-WALTER, Marie-Anne
dc.date.conference2018-09-10
dc.description.abstractEnIn Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Therefore, adaptation of new varieties to climate change is one challenging question for ongoing breeding programs. Efficient selection and vegetative propagation of improved varieties appeared to be key issues to maintain productivity in plantation forestry (Lelu-Walter et al. 2013). Somatic embryogenesis, coupled with cryopreservation, is a promising clonal propagation system of selected trees that has been successfully improved in Douglas-fir (Reeves et al. 2018; Lelu-Walter et al. 2018). Repetitive somatic embryogenesis from cotyledonary somatic embryos (SEs) has been obtained for two unrelated Douglas-fir genotypes, producing secondary and tertiary embryogenic lines. Each one exhibited significantly higher embryogenic potential for both genotypes compared to primary or secondary lines, respectively (increase of 63 to 727%). The origin of such differences in embryogenic potential is unknown. Our objective was to study changes induced in embryonal masses (EMs) after repetitive somatic embryogenesis at the histo-cytological and molecular levels (LC-MS-based proteomics). Repetitive somatic embryogenesis improved the EM structure by increasing frequency of small, singulated immature SEs together with reducing the size of polyembryogenic centers. Each cycle of embryogenesis induced a modification of the expression of proteins related with biological processes and already known to be involvedin somatic embryogenesis but quite new for EMs i.e.plant development, defense response, metabolism, proteolysis, and stress. The innovative implementation of protein networks in our proteomic analyses had been very conclusive. The networks revealed a global down or up pattern of protein expression after the first or second cycle of somatic embryogenesis, respectively. For both patterns, interactions with different plant growth signaling molecules (flavonoids and associated compounds, jasmonic acid, ABA, auxin, salicylic acid, lignin) were predominant. It is shown that cells have the ability to use different protein regulatory pathways to result in increased embryogenic potential (cotyledonary SE production). In Douglas-fir and in conifers, this is the first report describing cellular and molecular changes in EMs obtained after two successive cycles of repetitive somatic embryogenesis (Gautier et al. 2018). The results contribute to a better understanding of the cytological and proteomic changes associated with to enhanced embryogenic potential of secondary and tertiary embryogenic lines.
dc.language.isoen
dc.subject.enhistology
dc.subject.enembryogenic potential
dc.subject.enproteomic
dc.subject.enPseudotsuga menziesii
dc.subject.enplant growth regulators
dc.subject.ensecondary somatic embryogenesis
dc.title.enRepetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
dc.subject.halSciences du Vivant [q-bio]/Biologie du développement
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
bordeaux.pagep. 85
bordeaux.countryPT
bordeaux.conference.cityCoimbra
bordeaux.peerReviewednon
hal.identifierhal-03541954
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2018-09-15
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03541954v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.spage=p.%2085&rft.epage=p.%2085&rft.au=TEYSSIER,%20Caroline&GAUTIER,%20Florian&ELI%C3%81%C5%A0OV%C3%81,%20Kate%C5%99ina&LEPL%C3%89,%20Jean-Charles&VONDRAKOVA,%20Zuzana&rft.genre=unknown


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