RGD peptides micro-patterning on poly(ethylene terephthalate) surfaces
| hal.structure.identifier | Biomateriaux et Reparation Tissulaire | |
| dc.contributor.author | CHOLLET, Céline | |
| hal.structure.identifier | Laboratoire de Physico-Chimie Moléculaire [LPCM] | |
| dc.contributor.author | LAZARE, Sylvain | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | LABRUGÈRE, Christine | |
| hal.structure.identifier | Biomateriaux et Reparation Tissulaire | |
| dc.contributor.author | GUILLEMOT, Fabien | |
| hal.structure.identifier | Biomateriaux et Reparation Tissulaire | |
| dc.contributor.author | BAREILLE, Reine | |
| hal.structure.identifier | Biomateriaux et Reparation Tissulaire | |
| dc.contributor.author | DURRIEU, Marie-Christine | |
| dc.date.issued | 2007 | |
| dc.identifier.issn | 1297-9562 | |
| dc.description.abstractEn | he aim of this study was to evaluate the impact of RGD micro-patterned poly(ethylene terephthalate) (PET) on human osteoblast progenitor (HOP) cells attachment. Biomimetic modifications were performed by means of a four-step reaction: surface hydrolysis, oxidation in order to create COOH functions, coupling agent grafting (EDC, NHS) and finally immobilization of peptides. In addition to homogeneous or statistically distribution of peptides, micro-patterns of RGD were generated by: optical photolithography and UV excimer laser ablation. Modification steps were validated by physico-chemical techniques: XPS was used to prove covalent grafting at each stage of the surface functionalization, toluidine blue assay and high resolution µ-imager (using [3H]-Lys) to evaluate peptide densities and validate micro-patterns formation. Finally, the efficiency of this biomodification of PET was demonstrated onto homogeneous surfaces by measuring the adhesion between 1 and 24 h of osteoprogenitor cells isolated from HBMSC. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject.en | Biomaterials | |
| dc.subject.en | Grafting | |
| dc.subject.en | Micro-patterning | |
| dc.subject.en | RGD | |
| dc.title.en | RGD peptides micro-patterning on poly(ethylene terephthalate) surfaces | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.rbmret.2007.03.002 | |
| dc.subject.hal | Chimie/Matériaux | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biotechnologies | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Ingénierie biomédicale | |
| bordeaux.journal | ITBM RBM | |
| bordeaux.page | 2-12 | |
| bordeaux.volume | 28 | |
| bordeaux.issue | 1 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00185252 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00185252v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ITBM%20RBM&rft.date=2007&rft.volume=28&rft.issue=1&rft.spage=2-12&rft.epage=2-12&rft.eissn=1297-9562&rft.issn=1297-9562&rft.au=CHOLLET,%20C%C3%A9line&LAZARE,%20Sylvain&LABRUG%C3%88RE,%20Christine&GUILLEMOT,%20Fabien&BAREILLE,%20Reine&rft.genre=article |
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