CHOLLET, Céline; LABRUGÈRE, Christine; DURRIEU, Marie-Christine

doi:10.1016/j.rbmret.2007.12.003

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

CHOLLET, Céline
Biomateriaux et Reparation Tissulaire

LABRUGÈRE, Christine
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]

DURRIEU, Marie-Christine
Biomateriaux et Reparation Tissulaire

Langue

Article de revue

Ce document a été publié dans

ITBM RBM. 2008, vol. 29, n° 1, p. 7-12

Elsevier

Résumé en anglais

The aim of this study was to evaluate the impact of different densities on MC3T3 cells attachment onto polyethylene terephthalate (PET) film surfaces. Biomimetic modifications were performed by means of a three-step reaction procedure: creation of COOH functions onto PET surface, coupling agent grafting and finally immobilization of peptides. The originality of this work consist, in one hand on quantifying RGD peptides densities grafted onto PET, and on the other hand on studying MC3T3 cells responses after seeding on such biomimetic surfaces. After each functionnalization step, modifications were validated by several physicochemical techniques: X-Ray Photoelectron Spectroscopy permitted to prove the grafting and high-resolution β-imager coupled with use of radiolabelled amino acids served in evaluation of peptides densities. Moreover, this last technique permit us to ensure stability of binding between peptides and polymer. The efficiency of this new route for biomimetic modification of PET surface was demonstrated by measuring the adhesion at 15 hours of osteoblast like cells. Study of cellular comportment was realized by means of focal contact proteins (vinculin, actin) immunostaining.< Réduire

Mots clés en anglais

Biomaterials

Grafting

Polyethylene terephthalate

RGD peptides

Métadonnées

Licence d’utilisation du document

Impact of RGD peptide density grafted onto polyethylene terephthalate on MC3T3 cell attachment

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

DOI

Origine

Unités de recherche