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Nanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging.

hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierPlateforme Technologique et d'Innovation Biomédicale
dc.contributor.authorJACOBIN-VALAT, Marie-Josée
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierPlateforme Technologique et d'Innovation Biomédicale
dc.contributor.authorLAROCHE-TRAINEAU, Jeanny
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierPlateforme Technologique et d'Innovation Biomédicale
dc.contributor.authorLARIVIÈRE, Mélusine
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierPlateforme Technologique et d'Innovation Biomédicale
dc.contributor.authorSANCHEZ, Stephane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBIRAN, Marc
hal.structure.identifierBaculovirus et Thérapie
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorLEBARON, Caroline
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorBOUDON, Julien
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorLACOMME, Sabrina
hal.structure.identifierBaculovirus et Thérapie
hal.structure.identifierLaboratoire de Pathologie Comparée [LPC]
dc.contributor.authorCÉRUTTI, Martine
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
hal.structure.identifierPlateforme Technologique et d'Innovation Biomédicale
dc.contributor.authorCLOFENT-SANCHEZ, Gisèle
dc.date.issued2015
dc.identifier.issn1549-9634
dc.description.abstractEnAtherosclerosis is an inflammatory disease associated with the formation of atheroma plaques likely to rupture in which platelets are involved both in atherogenesis and atherothrombosis. The rupture is linked to the molecular composition of vulnerable plaques, causing acute cardiovascular events. In this study we propose an original targeted contrast agent for molecular imaging of atherosclerosis. Versatile USPIO (VUSPIO) nanoparticles, enhancing contrast in MR imaging, were functionalised with a recombinant human IgG4 antibody, rIgG4 TEG4, targeting human activated platelets. The maintenance of immunoreactivity of the targeted VUSPIO against platelets was confirmed in vitro by flow cytometry, transmission electronic and optical microscopy. In the atherosclerotic ApoE(-/-) mouse model, high-resolution ex vivo MRI demonstrated the selective binding of TEG4-VUSPIO on atheroma plaques. It is noteworthy that the rationale for targeting platelets within atherosclerotic lesions is highlighted by our targeted contrast agent using a human anti-αIIbβ3 antibody as a targeting moiety. Current clinical assessment of atherosclerotic plagues is suboptimal. The authors in the article designed functionalized superparamagnetic iron oxide nanoparticles with TEG4, a recombinant human antibody, to target activated platelets. By using MRI, these nanoparticles can be utilized to study the process of atheroma pathogenesis.
dc.description.sponsorshipTranslational Research and Advanced Imaging Laboratory - ANR-10-LABX-0057
dc.language.isoen
dc.publisherElsevier
dc.subject.enHuman antibody
dc.subject.enAtherosclerosis
dc.subject.enPlatelets
dc.subject.enNanoparticles
dc.subject.enMRI contrast agents
dc.title.enNanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.nano.2014.12.006
dc.subject.halChimie/Matériaux
bordeaux.journalNanomedicine: Nanotechnology, Biology and Medicine
bordeaux.page927-937
bordeaux.volume11
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-01228125
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01228125v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nanomedicine:%20Nanotechnology,%20Biology%20and%20Medicine&rft.date=2015&rft.volume=11&rft.issue=4&rft.spage=927-937&rft.epage=927-937&rft.eissn=1549-9634&rft.issn=1549-9634&rft.au=JACOBIN-VALAT,%20Marie-Jos%C3%A9e&LAROCHE-TRAINEAU,%20Jeanny&LARIVI%C3%88RE,%20M%C3%A9lusine&MORNET,%20St%C3%A9phane&SANCHEZ,%20Stephane&rft.genre=article


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