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Metallic oxide nanoparticle translocation across the human bronchial epithelial barrier.

hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorGEORGE, Isabelle
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorNAUDIN, Grégoire
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorBOLAND, Sonja
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierInstitut Jacques Monod [IJM (UMR_7592)]
dc.contributor.authorCONTREMOULINS, Vincent
hal.structure.identifierDirection de l'Action Sociale de l'Enfance et de la Santé [DASES]
dc.contributor.authorBEUGNON, Karine
hal.structure.identifierDirection de l'Action Sociale de l'Enfance et de la Santé [DASES]
dc.contributor.authorMARTINON, Laurent
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMBERT, Olivier
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorBAEZA-SQUIBAN, Armelle
dc.date.issued2015
dc.identifier.issn2040-3364
dc.description.abstractEnInhalation is the most frequent route of unintentional exposure to nanoparticles (NPs). Our aim was to quantify the translocation of different metallic NPs across human bronchial epithelial cells and to determine the factors influencing this translocation. Calu-3 cells forming a tight epithelial barrier when grown on a porous membrane in a two compartment chamber were exposed to fluorescently labelled NPs to quantify the NP translocation. NP translocation and uptake by cells were also studied by confocal and transmission electron microscopy. Translocation was characterized according to NP size (16, 50, or 100 nm), surface charge (negative or positive SiO2), composition (SiO2 or TiO2), presence of proteins or phospholipids and in an inflammatory context. Our results showed that NPs can translocate through the Calu-3 monolayer whatever their composition (SiO2 or TiO2), but this translocation was increased for the smallest and negatively charged NPs. Translocation was not associated with an alteration of the integrity of the epithelial monolayer, suggesting a transcytosis of the internalized NPs. By modifying the NP corona, the ability of NPs to cross the epithelial barrier differed depending on their intrinsic properties, making positively charged NPs more prone to translocate. NP translocation can be amplified by using agents known to open tight junctions and to allow paracellular passage. NP translocation was also modulated when mimicking an inflammatory context frequently found in the lungs, altering the epithelial integrity and inducing transient tight junction opening. This in vitro evaluation of NP translocation could be extended to other inhaled NPs to predict their biodistribution.
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.subject.ensilica nanoparticles
dc.subject.enCalu-3 cells
dc.subject.ennanoparticle uptake
dc.subject.enTranscytosis
dc.title.enMetallic oxide nanoparticle translocation across the human bronchial epithelial barrier.
dc.typeArticle de revue
dc.identifier.doi10.1039/c4nr07079h
dc.subject.halSciences du Vivant [q-bio]/Toxicologie
dc.subject.halChimie/Matériaux
bordeaux.journalNanoscale
bordeaux.page4529-4544
bordeaux.volume7
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-01228101
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01228101v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nanoscale&rft.date=2015&rft.volume=7&rft.issue=10&rft.spage=4529-4544&rft.epage=4529-4544&rft.eissn=2040-3364&rft.issn=2040-3364&rft.au=GEORGE,%20Isabelle&NAUDIN,%20Gr%C3%A9goire&BOLAND,%20Sonja&MORNET,%20St%C3%A9phane&CONTREMOULINS,%20Vincent&rft.genre=article


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